caa a22 4500 606234918 CHVBK 20210128101240.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2519-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2519-7 Effects of miRNA-145 on airway smooth muscle cells function [Elektronische Daten] [Yun Liu, Xiuzhen Sun, Yuanyuan Wu, Ping Fang, Hongyang Shi, Jing Xu, Manxiang Li] The pathological changes of airway smooth muscle (ASM) contribute to airway remodeling during asthma. Here, we investigated the effect of miR-145 on ASM function. We found that miR-145 was aberrantly more highly expressed in ASM cells exposed to cytokine stimulation that mimic the airway conditions of patients with asthma. Repression of miR-145 resulted in decreased ASM cell proliferation and migration in a dose-dependent manner and down-regulation of type I collagen and contractile protein MHC in ASM cells. qRT-PCR and Western blot analysis demonstrated that miR-145 negatively regulated the expression of downstream target Krüppel-like factor 4 (KLF4) protein, and overexpression of KLF4 attenuated the effects of miR-145 on ASM cells. Further studies showed that KLF4 significantly up-regulated the expression of p21 and down-regulated matrix metalloproteinase (MMP-2 and MMP-9). In conclusion, miR-145 overexpression in ASM cells significantly inhibited KLF4, and subsequently affected downstream p21, MMP-2, and MMP-9 expressions, eventually leading to enhanced proliferation and migration of ASM cells in vitro. Springer Science+Business Media New York, 2015 miRNA-145 nationallicence Airway smooth muscle cells nationallicence Airway remodeling nationallicence KLF4 nationallicence p21 nationallicence MMP-2 nationallicence Liu Yun Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Sun Xiuzhen Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Wu Yuanyuan Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Fang Ping Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Shi Hongyang Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Xu Jing Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Li Manxiang Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 135-143 0300-8177 409:1-2<135 2015 409 11010 https://doi.org/10.1007/s11010-015-2519-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2519-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yun Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 700 1- Sun Xiuzhen Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 700 1- Wu Yuanyuan Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 700 1- Fang Ping Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 700 1- Shi Hongyang Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 700 1- Xu Jing Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 700 1- Li Manxiang Department of Respiratory Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University Medical College, 157 Xiwu Road, 710004, Xi'an Shaanxi, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 135-143 0300-8177 409:1-2<135 2015 409 11010