caa a22 4500 606234926 CHVBK 20210128101240.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s11010-015-2516-x doi (NATIONALLICENCE)springer-10.1007/s11010-015-2516-x miR-25 promotes glioblastoma cell proliferation and invasion by directly targeting NEFL [Elektronische Daten] [Gang Peng, Xianrui Yuan, Jian Yuan, Qing Liu, Minhui Dai, Chenfu Shen, Jianrong Ma, Yiwei Liao, Weixi Jiang] Glioblastoma multiforme (GBM) is the most malignant and common brain tumor; it is aggressive growth pattern means that GBM patients face a poor prognosis even when receiving the best available treatment modalities. In recent years, an increasing number of reports suggest that the discovery of microRNAs (miRNAs) might provide a novel therapeutic target for human cancers, including GBM. One miRNA in particular, microRNA-25 (miR-25), is overexpressed in several cancers, wherein accumulating evidence indicates that it functions as an oncogene. However, the function of miR-25 in GBM has not been totally elucidated. In this study, we demonstrated that miR-25 was significantly up-regulated in astrocytoma tissues and glioblastoma cell lines. In vitro studies further demonstrated that overexpressed miR-25 was able to promote, while its antisense oligos inhibited cell proliferation and invasion in U251 cells. Moreover, we identified neurofilament light polypeptide (NEFL) as a novel target molecule of miR-25. Also of note was the fact that NEFL was down-regulated with increased levels of miR-25 expression in human astrocytoma clinical specimens. In addition, via the mTOR signaling pathway, NEFL-siRNA could significantly attenuate the inhibitory effects of knockdown miR-25 on the proliferation and invasion of U251 cells. Overall, our results showed an important role for miR-25 in regulating NEFL expression in GBM, and suggest that miR-25 could be a potential target for GBM treatment. Springer Science+Business Media New York, 2015 miR-25 nationallicence NEFL nationallicence Glioblastoma nationallicence Cell proliferation and invasion nationallicence Peng Gang Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Yuan Xianrui Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Yuan Jian Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Liu Qing Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Dai Minhui Department of Ophthalmology, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Shen Chenfu Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Ma Jianrong Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Liao Yiwei Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Jiang Weixi Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 103-111 0300-8177 409:1-2<103 2015 409 11010 https://doi.org/10.1007/s11010-015-2516-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2516-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Gang Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Yuan Xianrui Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Yuan Jian Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Liu Qing Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Dai Minhui Department of Ophthalmology, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Shen Chenfu Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Ma Jianrong Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Liao Yiwei Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Jiang Weixi Department of Neurosurgery, Xiangya Hospital of Central South University, 87 XiangYa Road, 410008, Changsha, Hunan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 409/1-2(2015-11-01), 103-111 0300-8177 409:1-2<103 2015 409 11010