caa a22 4500 606235051 CHVBK 20210128101240.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2206-0 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2206-0 Erythropoietin protects red blood cells from TRAIL1-induced cell death during red blood cell transition in Xenopus laevis [Elektronische Daten] [Kei Tamura, Nobuhiko Takamatsu, Michihiko Ito] In anuran amphibians, larval red blood cells (RBCs) are replaced by adult-type RBCs during metamorphosis. We previously showed that tumor necrosis factor-related apoptosis-inducing ligand 1 (TRAIL1) induces apoptosis in larval-, but not adult-type RBCs in Xenopus laevis. We also found that protein kinase C (PKC) activation is involved in establishing resistance to TRAIL1-induced apoptosis in adult-type RBCs. Here, we investigated whether erythropoietin (EPO), which induces PKC activation in mammalian erythroblasts, is involved in the RBC transition in X. laevis. RT-PCR analysis revealed that epo mRNA was upregulated in the lung, from the metamorphic climax (stage 60) onward. In an RBC culture system, EPO pretreatment significantly attenuated the TRAIL1-induced death of larval- and adult-type RBCs isolated from tadpoles and adults, probably due partly to PKC activation. In samples from froglets undergoing RBC transition, which included both larval- and adult-type RBCs, EPO exhibited a stronger protective effect on the adult-type than the larval-type RBCs. Newly differentiated RBCs isolated from tadpoles treated with a hemolytic reagent were more resistant to TRAIL1-induced cell death than non-treated controls. These results suggest that EPO functions to protect adult-type RBCs from TRAIL1-induced cell death during RBC transition, and that the protective effect might decrease as RBCs age. Springer Science+Business Media New York, 2014 Red blood cell transition nationallicence Erythropoietin nationallicence TRAIL nationallicence Protein kinase C nationallicence RBC : Red blood cell nationallicence EPO : Erythropoietin nationallicence TRAIL : Tumor necrosis factor-related apoptosis-inducing ligand nationallicence PKC : Protein kinase C nationallicence Tamura Kei Laboratory of Molecular Biology, Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Minamiku, 252-0373, Sagamihara, Japan aut Takamatsu Nobuhiko Laboratory of Molecular Biology, Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Minamiku, 252-0373, Sagamihara, Japan aut Ito Michihiko Laboratory of Molecular Biology, Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Minamiku, 252-0373, Sagamihara, Japan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 73-81 0300-8177 398:1-2<73 2015 398 11010 https://doi.org/10.1007/s11010-014-2206-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2206-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tamura Kei Laboratory of Molecular Biology, Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Minamiku, 252-0373, Sagamihara, Japan aut NATIONALLICENCE 700 1- Takamatsu Nobuhiko Laboratory of Molecular Biology, Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Minamiku, 252-0373, Sagamihara, Japan aut NATIONALLICENCE 700 1- Ito Michihiko Laboratory of Molecular Biology, Department of Biosciences, School of Science, Kitasato University, 1-15-1 Kitasato, Minamiku, 252-0373, Sagamihara, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 73-81 0300-8177 398:1-2<73 2015 398 11010