caa a22 4500 606235086 CHVBK 20210128101240.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2218-9 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2218-9 Modulation of inducible nitric oxide synthase (iNOS) expression and cardiovascular responses during static exercise following iNOS antagonism within the ventrolateral medulla [Elektronische Daten] [Pasarapa Towiwat, Siripan Phattanarudee, Timothy Maher, Ahmmed Ally] Previous reports indicate that inducible nitric oxide synthase (iNOS) blockade within the rostral ventrolateral medulla (RVLM) and caudal ventrolateral medulla (CVLM) differentially modulated cardiovascular responses, medullary glutamate, and GABA concentrations during static skeletal muscle contraction. In the current study, we determined the role of iNOS antagonism within the RVLM and CVLM on cardiovascular responses and iNOS protein expression during the exercise pressor reflex in anesthetized rats. Following 120min of bilateral microdialysis of a selective iNOS antagonist, aminoguanidine (AGN; 10µM), into the RVLM, the pressor responses were attenuated by 72% and changes in heart rate were reduced by 38% during a static muscle contraction. Furthermore, western blot analysis of iNOS protein abundance within the RVLM revealed a significant attenuation when compared to control animals. In contrast, bilateral administration of AGN (10µM) into the CVLM augmented the increases in mean arterial pressure by 60% and potentiated changes in heart rate by 61% during muscle contractions, but did not alter expression of the iNOS protein within the CVLM. These results demonstrate that iNOS protein expression within the ventrolateral medulla is differentially regulated by iNOS blockade that may, in part, contribute to the modulation of cardiovascular responses during static exercise. Springer Science+Business Media New York, 2014 Rostral ventrolateral medulla nationallicence Caudal ventrolateral medulla nationallicence Autonomic regulation nationallicence Microdialysis nationallicence Blood pressure nationallicence Nitric oxide nationallicence Towiwat Pasarapa Department of Pharmacology and Physiology, Chulalongkorn University, 10330, Bangkok, Thailand aut Phattanarudee Siripan Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 10330, Bangkok, Thailand aut Maher Timothy Department of Pharmaceutical Sciences, MCPHS University, 02115, Boston, MA, USA aut Ally Ahmmed Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Health Sciences University, 120 North Clovis Avenue, 93612, Clovis, CA, USA aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 185-194 0300-8177 398:1-2<185 2015 398 11010 https://doi.org/10.1007/s11010-014-2218-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2218-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Towiwat Pasarapa Department of Pharmacology and Physiology, Chulalongkorn University, 10330, Bangkok, Thailand aut NATIONALLICENCE 700 1- Phattanarudee Siripan Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, 10330, Bangkok, Thailand aut NATIONALLICENCE 700 1- Maher Timothy Department of Pharmaceutical Sciences, MCPHS University, 02115, Boston, MA, USA aut NATIONALLICENCE 700 1- Ally Ahmmed Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Health Sciences University, 120 North Clovis Avenue, 93612, Clovis, CA, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 185-194 0300-8177 398:1-2<185 2015 398 11010