caa a22 4500 606235094 CHVBK 20210128101240.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2227-8 doi (NATIONALLICENCE)springer-10.1007/s11010-014-2227-8 The effects of galectin-1 on the gene expression of the transcription factors TBX21, GATA-3, FOXP3 and RORC [Elektronische Daten] [V. Yakushina, O. Vasil'eva, N. Ryazantseva, V. Novitsky, L. Tashireva] CD4+ T cells orchestrate the immune response by differentiating into T helper (Th) or regulatory (Treg) cell subsets that secrete distinct sets of cytokines. They also play a critical role in the pathogenesis of autoimmunity, asthma, allergy and, likely, cancer. The mechanisms involved in the regulation of CD4+ T cell homeostasis by galectin-1 remain poorly characterized. To investigate whether galectin-1 modulates the differentiation of CD4+ T cells, the effects of galectin-1 on the mRNA expression levels of TBX21, GATA-3, FOXP3 and RORC in activated peripheral blood mononuclear cells were examined. The expression levels of GATA-3 and FOXP3 mRNA were up-regulated after treatment with 1.0μg/ml galectin-1 and were unchanged (for GATA-3) or slightly elevated (for FOXP3) compared with untreated cells when 2.0μg/ml galectin-1 was added. At the same time, at both concentrations of galectin-1, we observed reduced TBX21 and RORC mRNA expression levels. These findings support the concept that galectin-1 skews the differentiation of CD4+ T cells towards Th2 and Treg cells. Springer Science+Business Media New York, 2014 Galectin-1 nationallicence CD4+ T cells nationallicence Differentiation nationallicence Transcription factors nationallicence Yakushina V. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut Vasil'eva O. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut Ryazantseva N. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut Novitsky V. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut Tashireva L. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 245-249 0300-8177 398:1-2<245 2015 398 11010 https://doi.org/10.1007/s11010-014-2227-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2227-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yakushina V. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut NATIONALLICENCE 700 1- Vasil'eva O. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut NATIONALLICENCE 700 1- Ryazantseva N. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut NATIONALLICENCE 700 1- Novitsky V. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut NATIONALLICENCE 700 1- Tashireva L. Department of Molecular Medicine and Laboratory Medicine, Department of Pathophysiology, Science and Educational Center of Molecular Medicine, Siberian State Medical University, Tomsk, Russia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 245-249 0300-8177 398:1-2<245 2015 398 11010