caa a22 4500 606235108 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2215-z doi (NATIONALLICENCE)springer-10.1007/s11010-014-2215-z Dynamics of telopodes (telocyte prolongations) in cell culture depends on extracellular matrix protein [Elektronische Daten] [Cristina Niculite, T. Regalia, Mihaela Gherghiceanu, R. Huica, Mihaela Surcel, C. Ursaciuc, M. Leabu, L. Popescu] Telocytes (TC) are cells with telopodes (Tp), very long prolongations (up to 100μm) with an uneven caliber ( www.telocytes.com ). Factors determining the dynamics of cellular prolongations are still unknown, although previous studies showed telopode motility in TC cultures. We comparatively investigated, by time-lapse videomicroscopy, the dynamics of Tp of mouse heart TC seeded on collagen, fibronectin, and laminin. Under our experimental conditions, TC and fibroblasts (cell line L929) behaved differently in terms of adherence, spreading, and prolongation extension. Fibroblasts showed lower spreading on the matrix proteins used. The time needed for spreading was 2-4h for TC, versus 8-10h for fibroblasts. The values for final cell surface area after spreading were between 200 and 400μm2 for fibroblasts and 800-2,000μm2 for TC. TC showed a more than three times higher ability to spread on the tested matrix proteins. An extremely low capacity to extend prolongations with lengths shorter than cell bodies was noted for fibroblasts, while TC extended prolongations longer than the cell body length, with a moniliform appearance. The stronger adherence and spreading were noted for TC seeded on fibronectin, while the lowest were on laminin. Collagen determined an intermediate adherence and spreading for TC, but the highest dynamics in Tp extensions. In conclusion, TC behave differently than fibroblasts in terms of adherence, spreading, and cell prolongation extension when seeded on various matrix proteins in cell culture. The Author(s), 2014 Telocyte nationallicence Telopodes nationallicence Collagen nationallicence Fibronectin nationallicence Laminin nationallicence Time-lapse videomicroscopy nationallicence Cell layer impedance nationallicence Niculite Cristina Department of Cellular and Molecular Biology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut Regalia T. Department of Cellular and Molecular Biology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut Gherghiceanu Mihaela Laboratory of Electron Microscopy, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut Huica R. Department of Cellular and Molecular Medicine, "Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania aut Surcel Mihaela Department of Immunology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut Ursaciuc C. Department of Immunology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut Leabu M. Department of Cellular and Molecular Biology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut Popescu L. Department of Cellular and Molecular Medicine, "Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 157-164 0300-8177 398:1-2<157 2015 398 11010 https://doi.org/10.1007/s11010-014-2215-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2215-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Niculite Cristina Department of Cellular and Molecular Biology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut NATIONALLICENCE 700 1- Regalia T. Department of Cellular and Molecular Biology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut NATIONALLICENCE 700 1- Gherghiceanu Mihaela Laboratory of Electron Microscopy, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut NATIONALLICENCE 700 1- Huica R. Department of Cellular and Molecular Medicine, "Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania aut NATIONALLICENCE 700 1- Surcel Mihaela Department of Immunology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut NATIONALLICENCE 700 1- Ursaciuc C. Department of Immunology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut NATIONALLICENCE 700 1- Leabu M. Department of Cellular and Molecular Biology, "Victor Babeş” National Institute of Pathology, Bucharest, Romania aut NATIONALLICENCE 700 1- Popescu L. Department of Cellular and Molecular Medicine, "Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 157-164 0300-8177 398:1-2<157 2015 398 11010