caa a22 4500 606235159 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s11010-014-2224-y doi (NATIONALLICENCE)springer-10.1007/s11010-014-2224-y Disrupted hyaluronan binding protein 1 (HABP1) expression: one of the key mediator for ovarian dysfunction in polycystic ovary rat [Elektronische Daten] [Mohammed Arif, Sonu Thakur, Kasturi Datta] Proper follicular development is crucial for cumulus-oocyte complex (COC) maturation, ovulation and luteinisation. All these ovarian processes are regulated by finely tuned rapid tissue remodeling that involves hyaluronan and interconnecting hyaladherins-rich extracellular matrix synthesis and its breakdown by various proteinase systems like matrix metalloproteinase (MMP). Disrupted tissue remodeling machinery can result into pathophysiologies like atretic follicular cysts formation in polycystic ovary syndrome (PCOS). In present study, we employ superovulated (SO) and polycystic ovary (PCO) rat models and demonstrate that on contrary to SO, PCO rat ovary illustrates abnormal follicular morphology with differential levels of various ovarian factors [like HA (hyaluronan), TSG-6 (TNF-α-stimulated gene/protein 6), PTX-3 (pentraxin-3), HABP1 (hyaluronan binding protein 1), MMP2 (matrix metalloproteinase), MT1-MMP (membrane type 1-matrix metalloproteinase) and COX2 (Cyclooxygenase-2)] along with hyperactivities of gelatinases (like MMP9 and -2). Besides cultured COC expansion is blocked by anti-HABP1 antibody treatment showing reduced HABP1 expression. Overall, as MT1-MMP has inverse relation with HABP1 level and direct effect on MMP2 activity, the observations from current in vivo and in vitro studies indicate that disrupted ovarian HABP1 along with concurrent altered expression and hyperactivation of related MMPs can lead to abnormal follicular maturation resulting into ovarian dysfunction in PCO rat. Springer Science+Business Media New York, 2014 Polycystic ovary nationallicence COC nationallicence HABP1 nationallicence MMP nationallicence Arif Mohammed Biochemistry and Toxicology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut Thakur Sonu Reproductive Toxicology Laboratory, Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia University, 110025, New Delhi, India aut Datta Kasturi Biochemistry and Toxicology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 233-244 0300-8177 398:1-2<233 2015 398 11010 https://doi.org/10.1007/s11010-014-2224-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-014-2224-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arif Mohammed Biochemistry and Toxicology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut NATIONALLICENCE 700 1- Thakur Sonu Reproductive Toxicology Laboratory, Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia University, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Datta Kasturi Biochemistry and Toxicology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, 110067, New Delhi, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 398/1-2(2015-01-01), 233-244 0300-8177 398:1-2<233 2015 398 11010