caa a22 4500 606235205 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2503-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2503-2 Effect of ageing and oxidative stress on antioxidant enzyme activity in different regions of the rat kidney [Elektronische Daten] [Noor Thiab, Nicola King, Graham Jones] Oxidative stress has been implicated in ageing and the pathogenesis of chronic kidney disease. We examined levels of antioxidant enzymes glutathione peroxidase, glutathione reductase, glutathione S-transferase, catalase and superoxide dismutase as modulated by age and oxidative stress in different regions of the kidney. Antioxidant enzymes were examined in different regions of the kidney in male Wistar rats. Kidneys from rats of different ages (5, 12, 36 and 60weeks) were dissected into cortex, outer medulla and inner medulla. Tissues were incubated for 30min with or without 0.2mM H2O2 to induce oxidative stress. Antioxidant enzyme activities progressively decreased with age under both control and stress conditions (P<0.05) after peaking at 12weeks. Antioxidant enzyme activities were greater in the cortex (P<0.05) by comparison with the outer and inner medulla, respectively. Springer Science+Business Media New York, 2015 Male Wistar rats nationallicence Ageing nationallicence Oxidative stress nationallicence Antioxidant enzymes nationallicence Kidney regions nationallicence Thiab Noor Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, 2351, Armidale, NSW, Australia aut King Nicola School of Biomedical and Healthcare Sciences, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth University, PL4 8AA, Plymouth, UK aut Jones Graham Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, 2351, Armidale, NSW, Australia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 253-260 0300-8177 408:1-2<253 2015 408 11010 https://doi.org/10.1007/s11010-015-2503-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2503-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thiab Noor Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, 2351, Armidale, NSW, Australia aut NATIONALLICENCE 700 1- King Nicola School of Biomedical and Healthcare Sciences, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth University, PL4 8AA, Plymouth, UK aut NATIONALLICENCE 700 1- Jones Graham Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, 2351, Armidale, NSW, Australia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 253-260 0300-8177 408:1-2<253 2015 408 11010