caa a22 4500 60623523X CHVBK 20210128101241.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2482-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2482-3 Effects of ageing on metabolite and oxidant concentrations in different regions of rat kidney under normal and stress conditions [Elektronische Daten] [Noor Thiab, Nicola King, Graham Jones] Metabolic and oxidative stresses have been implicated in ageing and the pathogenesis of chronic kidney disease. In this study, we investigated the glutathione (GSH), thiobarbituric acid reactive substances (TBARS) and lactate concentrations in different kidney regions under control conditions and after exposure to oxidative stress invoked by 0.2mM H2O2. Slices of superficial cortex, outer or inner medulla were dissected from kidneys of male Wistar rats of 5-, 12-, 36- and 60-week old. Samples were incubated for 30min±0.2mM H2O2 prior to homogenisation and centrifugation. The concentrations of GSH, TBARS and lactate were measured by colorimetry. Each metabolite showed a distinctive pattern. For GSH, this was 12weeks>36weeks>60weeks and 5weeks with the highest concentration measured in the superficial cortex at 12weeks. For TBARS and lactate, the pattern was for the lowest concentration at 12weeks and the highest at 60 and 5weeks. The highest lactate and TBARS concentrations were measured under oxidative stress conditions, particularly at 5 and 60weeks. These results suggest that GSH in different kidney regions peaks at maturity and then reduces with increasing age. Springer Science+Business Media New York, 2015 Ageing nationallicence Kidney region nationallicence Oxidative stress and lactate nationallicence GSH : Glutathione nationallicence TBARS : Thiobarbituric acid reactive species nationallicence ROS : Reactive oxygen species nationallicence $${\text{O}}_{2}^{ - \cdot}$$ O 2 - · : Superoxide nationallicence OH· : Hydroxyl radical nationallicence $${\text{O}}_{2}^{1}$$ O 2 1 : Singlet oxygen nationallicence GSSG : Oxidised glutathione nationallicence MDA : Malondialdehyde nationallicence PBS : Phosphate buffered saline nationallicence BSA : Bovine serum albumin nationallicence Thiab Noor School of Science and Technology, Centre for Bioactive Discovery in Health and Ageing, University of New England, 2351, Armidale, NSW, Australia aut King Nicola School of Biomedical and Healthcare Sciences, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth University, PL4 8AA, Plymouth, UK aut Jones Graham School of Science and Technology, Centre for Bioactive Discovery in Health and Ageing, University of New England, 2351, Armidale, NSW, Australia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 55-61 0300-8177 408:1-2<55 2015 408 11010 https://doi.org/10.1007/s11010-015-2482-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2482-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Thiab Noor School of Science and Technology, Centre for Bioactive Discovery in Health and Ageing, University of New England, 2351, Armidale, NSW, Australia aut NATIONALLICENCE 700 1- King Nicola School of Biomedical and Healthcare Sciences, Plymouth University Peninsula Schools of Medicine and Dentistry, Plymouth University, PL4 8AA, Plymouth, UK aut NATIONALLICENCE 700 1- Jones Graham School of Science and Technology, Centre for Bioactive Discovery in Health and Ageing, University of New England, 2351, Armidale, NSW, Australia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 55-61 0300-8177 408:1-2<55 2015 408 11010