caa a22 4500 606235248 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2483-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2483-2 Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats [Elektronische Daten] [Suresh Gupta, Shirish Dongare, Rajani Mathur, Ipseeta Mohanty, Sushma Srivastava, Sandeep Mathur, Tapas Nag] The present study was undertaken to evaluate the protective effects of genistein against cardiac inflammation and oxidative stress in streptozotocin (STZ) (45mg/kg body weight)-induced diabetic rats. genistein (300mg/kg/day) was administered orally for 24weeks to STZ-induced diabetic rats. The effects of genistein on blood glucose, % glycosylated hemoglobin (HbA1c), C-reactive protein, tumor necrosis factor (TNF- α), transforming growth factor (TGF-β1), and total antioxidant were studied. Ultrastructural and histopathological assessment of injury were also undertaken using transmission electron microscope. STZ-induced diabetes resulted in significant increase in the levels of blood glucose, HbA1c, C-reactive protein, TNF- α and TGF-β1, and a decline in total antioxidant reserve of the myocardium. Administration of genistein to diabetic rats resulted in a decrease in blood glucose (p<0.001), % HbA1c (p<0.0001), C-reactive protein (p<0.001), and expression of TNF- α (p<0.001) and TGF-β1 (p<0.0001) proteins. In addition, genistein treatment results in augmentation of total antioxidant (p<0.01) reserve of the hearts. The above findings were supported by histological as well as immunohistochemical localization of NF-κB (p65) in the heart. Genistein treatment ameliorated the ultrastructural degenerative changes in the cardiac tissues as compared to the diabetic control. The result demonstrates that genistein restored the integrity of the diabetic myocardium by virtue of its anti-inflammatory and antioxidant effects. Springer Science+Business Media New York, 2015 Diabetic cardiomyopathy nationallicence Genistein nationallicence Oxidative stress nationallicence Inflammation nationallicence Gupta Suresh Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut Dongare Shirish Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut Mathur Rajani Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut Mohanty Ipseeta Departments of Pharmacology, MGM Medical College, Navi Mumbai, India aut Srivastava Sushma Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut Mathur Sandeep Department of Pathology, All India Institute of Medical Sciences, New Delhi, India aut Nag Tapas Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 63-72 0300-8177 408:1-2<63 2015 408 11010 https://doi.org/10.1007/s11010-015-2483-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2483-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gupta Suresh Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut NATIONALLICENCE 700 1- Dongare Shirish Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut NATIONALLICENCE 700 1- Mathur Rajani Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut NATIONALLICENCE 700 1- Mohanty Ipseeta Departments of Pharmacology, MGM Medical College, Navi Mumbai, India aut NATIONALLICENCE 700 1- Srivastava Sushma Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, 110017, New Delhi, India aut NATIONALLICENCE 700 1- Mathur Sandeep Department of Pathology, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 700 1- Nag Tapas Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 63-72 0300-8177 408:1-2<63 2015 408 11010