caa a22 4500 606235256 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2484-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2484-1 Anti-cancer effects of enteric-coated polymers containing mistletoe lectin in murine melanoma cells in vitro and in vivo [Elektronische Daten] [Seung-Yeon Han, Chang-Eui Hong, Hwan-Gyu Kim, Su-Yun Lyu] In this study, we evaluated the effects of Korean mistletoe (Viscum album L. var. coloratum) coated with a biodegradable polymer (Eudragit®) wall on the growth of mouse melanoma in vivo. Oral administration of 4% (430mg/kg/day) enteric-coated mistletoe resulted in a significant reduction in tumor volume on day 14 compared to the negative control group in B16F10 melanoma-inoculated BDF1 mice. When we measured the survival rate, enteric-coated mistletoe-received mice had a higher survival rate after day 12. Also, we investigated the mechanism involving the cancer cell growth inhibition when melanoma cells were treated with Korean mistletoe lectin (Viscum album L. var. coloratum agglutinin, VCA) and its extract in vitro. As a result, a significant G0/G1 arrest was observed in both B16BL6 and B16F10 melanoma cells with VCA or mistletoe extract. In addition, VCA or mistletoe extract induced an increase in both early and late apoptosis in cells. When we studied the molecular mechanism, our results showed that VCA and mistletoe extract can increase activated multiple caspases (caspase-1, 3, 4, 5, 6, 7, 8, and 9), dose-dependently. We also found out that VCA and mistletoe treatment causes a significant decrease in the expression of procaspase-3 and 8. Springer Science+Business Media New York, 2015 Mistletoe lectin nationallicence Melanoma nationallicence Apoptosis nationallicence Enteric coating nationallicence Han Seung-Yeon Department of Pharmacy, Sunchon National University, 255, Jungangno, 540-742, Suncheon, Jeonnam, Korea aut Hong Chang-Eui Department of Biology, Chonbuk National University, 567, Baekje-daero, Deokjin-gu, 561-756, Jeonju-si, Jeonbuk, Korea aut Kim Hwan-Gyu Department of Biology, Chonbuk National University, 567, Baekje-daero, Deokjin-gu, 561-756, Jeonju-si, Jeonbuk, Korea aut Lyu Su-Yun Department of Pharmacy, Sunchon National University, 255, Jungangno, 540-742, Suncheon, Jeonnam, Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 73-87 0300-8177 408:1-2<73 2015 408 11010 https://doi.org/10.1007/s11010-015-2484-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2484-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Han Seung-Yeon Department of Pharmacy, Sunchon National University, 255, Jungangno, 540-742, Suncheon, Jeonnam, Korea aut NATIONALLICENCE 700 1- Hong Chang-Eui Department of Biology, Chonbuk National University, 567, Baekje-daero, Deokjin-gu, 561-756, Jeonju-si, Jeonbuk, Korea aut NATIONALLICENCE 700 1- Kim Hwan-Gyu Department of Biology, Chonbuk National University, 567, Baekje-daero, Deokjin-gu, 561-756, Jeonju-si, Jeonbuk, Korea aut NATIONALLICENCE 700 1- Lyu Su-Yun Department of Pharmacy, Sunchon National University, 255, Jungangno, 540-742, Suncheon, Jeonnam, Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 73-87 0300-8177 408:1-2<73 2015 408 11010