caa a22 4500 606235299 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2489-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2489-9 SF - 1 (NR5A1) expression is stimulated by the PKA pathway and is essential for the PKA-induced activation of LIPE expression in Y-1 cells [Elektronische Daten] [K. Kulcenty, M. Holysz, W. Trzeciak] In the adrenal cortex, corticotropin induces the expression of several genes encoding proteins involved in the synthesis and intracellular transport of steroid hormones via the protein kinase A (PKA) signalling pathway, and this process is mediated by steroidogenic factor-1 (SF-1). This study was designed to elucidate the influence of the PKA and PKC pathways on the expression of the SF-1 gene in mouse adrenocortical cells, line Y-1. It has also been attempted to answer the question whether or not SF-1 plays a role in the PKA-induced expression of LIPE gene encoding hormone-sensitive lipase/cholesteryl esterase, which supplies cholesterol for steroid hormone synthesis. In this study, we found that stimulation of the PKA pathway caused a significant increase in SF-1 expression, and that this effect was abolished by the PKA inhibitor, H89. Decreased SF-1 gene transcript levels were seen with the simultaneous activation of PKA and PKC, suggesting a possible interaction between the PKA and PKC pathways. It was also observed that SF-1 increased the transcriptional activity of the LIPE gene by interacting with the SF-1 response element located in promoter A. Moreover, transient silencing of SF-1 expression with specific siRNAs abolished PKA-stimulated transcription of the LIPE gene, indicating that SF-1 is an important regulator of LIPE expression in Y-1 cells and thus could play a role in the regulation of the cholesterol supply for adrenal steroidogenesis. The Author(s), 2015 SF-1 nationallicence PKA nationallicence LIPE nationallicence HSL nationallicence Y-1 cells nationallicence Kulcenty K. Department of Cancer Immunology, Poznan University of Medical Sciences, Poznan, Poland aut Holysz M. Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781, Poznan, Poland aut Trzeciak W. Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781, Poznan, Poland aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 139-145 0300-8177 408:1-2<139 2015 408 11010 https://doi.org/10.1007/s11010-015-2489-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2489-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kulcenty K. Department of Cancer Immunology, Poznan University of Medical Sciences, Poznan, Poland aut NATIONALLICENCE 700 1- Holysz M. Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781, Poznan, Poland aut NATIONALLICENCE 700 1- Trzeciak W. Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 6 Swiecickiego St., 60-781, Poznan, Poland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 139-145 0300-8177 408:1-2<139 2015 408 11010