caa a22 4500 606235302 CHVBK 20210128101241.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2492-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2492-1 Myricetin inhibits proliferation and induces apoptosis and cell cycle arrest in gastric cancer cells [Elektronische Daten] [Jianfang Feng, Xiaonan Chen, Yuanyuan Wang, Yuwen Du, Qianqian Sun, Wenqiao Zang, Guoqiang Zhao] Myricetin is a flavonoid that is abundant in fruits and vegetables and has protective effects against cancer and diabetes. However, the mechanism of action of myricetin against gastric cancer (GC) is not fully understood. We researched myricetin on the proliferation, apoptosis, and cell cycle in GC HGC-27 and SGC7901 cells, to explore the underlying mechanism of action. Cell Counting Kit (CCK)-8 assay, Western blotting, cell cycle analysis, and apoptosis assay were used to evaluate the effects of myricetin on cell proliferation, apoptosis, and the cell cycle. To analyze the binding properties of ribosomal S6 kinase 2 (RSK2) with myricetin, surface plasmon resonance (SPR) analysis was performed. CCK8 assay showed that myricetin inhibited GC cell proliferation. Flow cytometry analysis showed that myricetin induces apoptosis and cell cycle arrest in GC cells. Western blotting indicated that myricetin influenced apoptosis and cell cycle arrest of GC cells by regulating related proteins. SPR analysis showed strong binding affinity of RSK2 and myricetin. Myricetin bound to RSK2, leading to increased expression of Mad1, and contributed to inhibition of HGC-27 and SGC7901 cell proliferation. Our results suggest the therapeutic potential of myricetin in GC. Springer Science+Business Media New York, 2015 Myricetin nationallicence Gastric cancer nationallicence Ribosomal S6 kinase 2 nationallicence Mad1 nationallicence Feng Jianfang Medical College of Henan University of Science and Technology, 471003, Luoyang, China aut Chen Xiaonan College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut Wang Yuanyuan College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut Du Yuwen College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut Sun Qianqian College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut Zang Wenqiao College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut Zhao Guoqiang College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 163-170 0300-8177 408:1-2<163 2015 408 11010 https://doi.org/10.1007/s11010-015-2492-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2492-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Feng Jianfang Medical College of Henan University of Science and Technology, 471003, Luoyang, China aut NATIONALLICENCE 700 1- Chen Xiaonan College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Wang Yuanyuan College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Du Yuwen College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Sun Qianqian College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Zang Wenqiao College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Zhao Guoqiang College of Basic Medical Sciences, Zhengzhou University, No. 100 Kexue Road, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 163-170 0300-8177 408:1-2<163 2015 408 11010