caa a22 4500 606235337 CHVBK 20210128101242.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2478-z doi (NATIONALLICENCE)springer-10.1007/s11010-015-2478-z SRD5A2 gene expression inhibits cell migration and invasion in prostate cancer cell line via F-actin reorganization [Elektronische Daten] [Suruchi Aggarwal, Minu Singh, Ashok Kumar, Tapas Mukhopadhyay] Steroid 5-alpha reductase type 2 (SRD5A2) gene is important for normal development and functioning of prostate gland but it is reported to be silenced in metastatic prostate cancer. We showed that exogenous SRD5A2 expression in prostate cancer cell line reduced cell migration and invasion. Additionally, the stable transfectants showed enhanced adhesion to the matrix accompanied by changes in cytoskeletal organization, involving actin polymerization. siRNA knockdown of the endogenous SRD5A2 mRNA in LnCAP cells was effective, it reversed the phenotype, and thus induced cell motility. The MEK1 and pERK1/2 levels were found to be reduced in SRD5A2-expressing cells. Further, the reduced level of p38 protein was correlated with low expression of MMP-2 and MMP-7 genes. The results suggest that SRD5A2 controls cell migration by indirectly regulating ERK/MAPK pathway. Springer Science+Business Media New York, 2015 SRD5A2 nationallicence Prostate cancer nationallicence Migration nationallicence Invasion nationallicence Actin cytoskeleton nationallicence Aggarwal Suruchi National Centre for Human Genome Studies and Research, Panjab University, 160014, Chandigarh, India aut Singh Minu National Centre for Human Genome Studies and Research, Panjab University, 160014, Chandigarh, India aut Kumar Ashok Department of Systems Biology, Panjab University, Chandigarh, India aut Mukhopadhyay Tapas National Centre for Human Genome Studies and Research, Panjab University, 160014, Chandigarh, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 15-23 0300-8177 408:1-2<15 2015 408 11010 https://doi.org/10.1007/s11010-015-2478-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2478-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aggarwal Suruchi National Centre for Human Genome Studies and Research, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Singh Minu National Centre for Human Genome Studies and Research, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 700 1- Kumar Ashok Department of Systems Biology, Panjab University, Chandigarh, India aut NATIONALLICENCE 700 1- Mukhopadhyay Tapas National Centre for Human Genome Studies and Research, Panjab University, 160014, Chandigarh, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 15-23 0300-8177 408:1-2<15 2015 408 11010