caa a22 4500 606235353 CHVBK 20210128101242.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2479-y doi (NATIONALLICENCE)springer-10.1007/s11010-015-2479-y Urinary monocyte chemoattractant protein-1 and vitamin D-binding protein as biomarkers for early detection of diabetic nephropathy in type 2 diabetes mellitus [Elektronische Daten] [Amira Shoukry, Shereen Bdeer, Rehab El-Sokkary] Diabetic nephropathy is a serious complication of both type 1 and type 2 diabetes and, unless arrested, leads to end-stage renal disease. Therefore, early prediction and detection of DN would greatly benefit the disease management and delay its progression. The aim of this study is to evaluate the levels of urinary monocyte chemoattractant protein-1 (uMCP-1) and urinary vitamin D-binding protein (uVDBP) in type 2 diabetic patients with different degrees ofdiabetic nephropathy (DN) and to assess the value of uMCP-1 and uVDBP in the early detection of DN. Seventy-five type 2 diabetic patients with normoalbuminuria (n=25), microalbuminuria (n=25), macroalbuminuria (n=25), and 25 healthy controls were included in this study. Urinary MCP-1 and VDBP levels were evaluated by enzyme-linked immunosorbent assay. A significant elevation in the uMCP-1 and uVDBP levels was found in macroalbuminuric (p<0.001) and microalbuminuric (p<0.01) diabetic patients compared to that in normoalbuminuric diabetic patients and control subjects (p<0.001). Correlation study revealed that both uMCP-1 and uVDBP were significantly positively correlated to urinary albumin/creatinine ratio (r=0.968, p<0.001 and r=0.973, p<0.001, respectively), serum urea (r=0.461,p=0.001 and r=0.456, p=0.002, respectively), and serum creatinine (r=0.475, p=0.001 and r=0.448, p=0.004, respectively) and significantly inversely correlated to glomerular filtration rate (r=−0.983, p<0.001 and r=−0.988, p<0.001, respectively). Receiver operating characteristic(ROC) curve analysis of uMCP-1 and uVDBP levels for early diagnosis and detection of DN revealed that the cut-off value of uMCP-1 was 110pg/mg with 92% sensitivity and 100% specificity; whereas, the cut-off value of uVDBP was 550ng/mg with 96% sensitivity and 84% specificity. The findings of the present study suggest that uMCP-1 and uVDBP may be considered as novel potential diagnostic biomarkers for the early detection of diabetic nephropathy. Springer Science+Business Media New York, 2015 Diabetic nephropathy nationallicence Urinary monocyte chemoattractant protein-1 nationallicence Urinary vitamin D-binding protein nationallicence Shoukry Amira Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Bdeer Shereen Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut El-Sokkary Rehab Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 25-35 0300-8177 408:1-2<25 2015 408 11010 https://doi.org/10.1007/s11010-015-2479-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2479-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shoukry Amira Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 700 1- Bdeer Shereen Physiology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 700 1- El-Sokkary Rehab Medical Microbiology and Immunology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 25-35 0300-8177 408:1-2<25 2015 408 11010