caa a22 4500 606235396 CHVBK 20210128101242.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2490-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2490-3 LPA signaling through LPA receptors regulates cellular functions of endothelial cells treated with anticancer drugs [Elektronische Daten] [Shiori Mori, Mutsumi Araki, Shuhei Ishii, Miku Hirane, Kaori Fukushima, Ayaka Tomimatsu, Kaede Takahashi, Nobuyuki Fukushima, Toshifumi Tsujiuchi] Lysophosphatidic acid (LPA) signaling via LPA receptors provides a variety of cellular functions, including angiogenesis. In this study, to assess an involvement of LPA receptors in cell motile activities of endothelial cells during chemotherapy, F-2 cells were treated with cisplatin (CDDP) and doxorubicin (DOX) at a concentration of 0.01μM every 24h for at least 1month. The treatment of CDDP and DOX inhibited the expression levels of the LPA receptor-1 (Lpar1), Lpar2, and Lpar3 genes in F-2 cells. The cell motile activities of CDDP and DOX treated cells were relatively lower than those of untreated cells. Next, we investigated whether cancer cells could stimulate the cell motile activities of F-2 cells treated with CDDP and DOX. For cell motility assay, CDDP- and DOX-treated cells were co-cultured with pancreatic cancer PANC-1 cells. The cell motile activities of CDDP- and DOX-treated cells were significantly enhanced by the existence of PANC-1 cells, correlating with the LPA receptor expressions. In addition, the elevated cell motile activities were suppressed by the pretreatment of an autotaxin inhibitor S32826. These results suggest that LPA signaling via LPA receptors may regulate the cell motile activities of F-2 cells treated with anticancer drugs. Springer Science+Business Media New York, 2015 Lysophosphatidic acid nationallicence LPA receptor nationallicence Cell motility nationallicence Endothelial cells nationallicence Anticancer drugs nationallicence Mori Shiori Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Araki Mutsumi Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Ishii Shuhei Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Hirane Miku Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Fukushima Kaori Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Tomimatsu Ayaka Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Takahashi Kaede Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Fukushima Nobuyuki Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Tsujiuchi Toshifumi Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 147-154 0300-8177 408:1-2<147 2015 408 11010 https://doi.org/10.1007/s11010-015-2490-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2490-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mori Shiori Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Araki Mutsumi Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Ishii Shuhei Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Hirane Miku Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Fukushima Kaori Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Tomimatsu Ayaka Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Takahashi Kaede Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Fukushima Nobuyuki Division of Molecular Neurobiology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 700 1- Tsujiuchi Toshifumi Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kinki University, 3-4-1, Kowakae, 577-8502, Higashiosaka, Osaka, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 147-154 0300-8177 408:1-2<147 2015 408 11010