caa a22 4500 606235477 CHVBK 20210128101242.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s11010-015-2497-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2497-9 Angiotensin II increases secreted frizzled-related protein 5 (sFRP5) expression through AT1 receptor/Rho/ROCK1/JNK signaling in cardiomyocytes [Elektronische Daten] [Xin Jin, Bingyan Guo, Jie Yan, Rong Yang, Liang Chang, Yaling Wang, Chenglong Miao, Suyun Liu, Hui Zhang, Yongjun Li] Secreted frizzled-related protein 5 (sFRP5) is a novel adipokine that functions as an inhibitor of Wnt signaling and is involved in embryonic development, proliferation, atherosclerosis, and apoptosis. Studies have shown that sFRP1-4 is expressed in cardiomyocytes, and sFRP3 and sFRP4are elevated during heart failure. However, it is unclear whether sFRP5 is expressed in cardiomyocytes or cardiac hypertrophy, and as regards the effects of sFRP5 in the process. Here, we report the expression and the corresponding mechanisms of sFRP5 in angiotensin II (Ang II)-induced cardiomyocyte hypertrophy. Neonatal rat ventricular myocytes were exposed to increasing concentrations of Ang II for 12-72h. Y27632 was used to block ROCK signal. PD98059, SB203580, and SP600125 were used to inhibit ERK1/2, p38 MAPK, and JNK signaling pathways, respectively, and anisomycin was used to activate JNK pathway. RT-PCR and Western-blot determined the expressions of sFRP5. BNP, TNF-α, ROCK1, ROCK2, MYPT1, and JNK were examined through Western-blot analysis. Ang II increased sFRP5 mRNA and protein levels in a time- and dose-dependent manner. Telmisartan, Y27632 and SP600125 effectively suppressed the expression of sFRP5. sFRP5 downregulated BNP and TNF-α expressions in hypertrophic cardiomyocytes. sFRP5 is expressed in cardiomyocytes, and upregulated in Ang II-induced cardiomyocyte hypertrophy through the AT1 receptor/Rho/ROCK1/JNK signaling pathway. sFRP5 may play an important role during cardiomyocyte hypertrophy. Springer Science+Business Media New York, 2015 Secreted frizzled-related protein 5 nationallicence Angiotensin II nationallicence Cardiomyocyte nationallicence Hypertrophy nationallicence Adipokine nationallicence Jin Xin Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Guo Bingyan Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Yan Jie Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Yang Rong Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Chang Liang Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Wang Yaling Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Miao Chenglong Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Liu Suyun Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Zhang Hui Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Li Yongjun Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 215-222 0300-8177 408:1-2<215 2015 408 11010 https://doi.org/10.1007/s11010-015-2497-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2497-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jin Xin Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Guo Bingyan Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Yan Jie Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Yang Rong Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Chang Liang Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Wang Yaling Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Miao Chenglong Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Liu Suyun Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Zhang Hui Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 700 1- Li Yongjun Department of Cardiovascular Medicine, The Second Hospital of Hebei Medical University, 050000, Shijiazhuang, Hebei, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 408/1-2(2015-10-01), 215-222 0300-8177 408:1-2<215 2015 408 11010