caa a22 4500 606235507 CHVBK 20210128101242.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2468-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2468-1 Effect of estrogen on expression of prohibitin in white adipose tissue and liver of diet-induced obese rats [Elektronische Daten] [Minji Choi, Harmesh Chaudhari, Young Ji, Zae Ryoo, Sang Kim, Jong Yun] Prohibitin (PHB) is a ubiquitously expressed and highly conserved protein that participates in diverse cellular processes, and its functions are linked to a variety of diseases. In the present study, to explore transcriptional activation and signaling pathways involved in PHB regulation in response to sex hormone treatment, we investigated the effects of estrogen (17-β-estradiol, E2) on regulation of PHB in several metabolic tissues from male and female rats. Elevated expression of PHB was prominent in white adipose tissue (WAT) and the liver, and E2 stimulated PHB expression in both ND and HFD-fed rats. To further confirm the expression of PHB which was increased in WAT and the liver, we analyzed PHB expression levels in 3T3-L1 and C9 cells after the treatment of E2. Transcription and protein levels of PHB were dose-dependently increased by E2 treatment in both cell types, supporting our in vivo data. To further evaluate the possible role of E2 in elevation of PHB via estrogen receptors (ER), the potent ER inhibitor fulvestrant was treated to 3T3-L1 and C9 cells. Fulvestrant markedly suppressed both transcription and protein levels of PHB, suggesting that PHB expression in both tissues may be regulated through ERs. GeneMANIA, a predictive web interface, was used to show that Phb is regulated via the intracellular steroid hormone receptor signaling pathway, suggesting a role for ERs in expression of Phb as well as other metabolically important genes. Based on these results, we expect that targeting PHB would be a useful therapeutic approach for treatment of obesity. Springer Science+Business Media New York, 2015 Adipose tissue nationallicence Estrogen receptor nationallicence Obesity nationallicence Prohibitin nationallicence Sex hormone nationallicence ANXA2/ Anxa2 : Annexin A2/-encoding gene nationallicence ARL4A/ Arl4a : ADP-ribosylation factor-like protein 4A/-encoding gene nationallicence AR : Androgen receptor nationallicence BAT : Brown adipose tissue nationallicence CON : Control nationallicence ER : Estrogen receptor nationallicence E2 : 17-β-Estradiol nationallicence Esr1 : Estrogen receptor α-encoding gene nationallicence Esr2 : Estrogen receptor β-encoding gene nationallicence LP3/ Gzmb : Granzyme-like protein 3/-encoding gene nationallicence HFD : High fat diet nationallicence IL17RB/ Il17rb : Interleukin-17 receptor B/-encoding gene nationallicence MATK/ Matk : Megakaryocyte-associated tyrosine kinase/-encoding gene nationallicence ND : Normal diet nationallicence PHB/ Phb : Prohibitin/-encoding gene nationallicence PHB2/ Phb2 : Prohibitin 2/-encoding gene nationallicence USP15/ Usp15 : Ubiquitin carboxyl-terminal hydrolase 15/-encoding gene nationallicence WAT : White adipose tissue nationallicence Choi Minji Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut Chaudhari Harmesh Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut Ji Young School of Life Science and Biotechnology, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Ryoo Zae School of Life Science and Biotechnology, Kyungpook National University, 702-701, Daegu, Republic of Korea aut Kim Sang Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut Yun Jong Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 181-196 0300-8177 407:1-2<181 2015 407 11010 https://doi.org/10.1007/s11010-015-2468-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2468-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Minji Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut NATIONALLICENCE 700 1- Chaudhari Harmesh Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut NATIONALLICENCE 700 1- Ji Young School of Life Science and Biotechnology, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Ryoo Zae School of Life Science and Biotechnology, Kyungpook National University, 702-701, Daegu, Republic of Korea aut NATIONALLICENCE 700 1- Kim Sang Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut NATIONALLICENCE 700 1- Yun Jong Department of Biotechnology, Daegu University, 712-714, Kyungsan, Kyungbuk, Republic of Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 181-196 0300-8177 407:1-2<181 2015 407 11010