caa a22 4500 606235531 CHVBK 20210128101243.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2450-y doi (NATIONALLICENCE)springer-10.1007/s11010-015-2450-y Functions of miR-1 and miR-133a during the postnatal development of masseter and gastrocnemius muscles [Elektronische Daten] [Megumi Nariyama, Manami Mori, Emi Shimazaki, Hitoshi Ando, Yoshiki Ohnuki, Tokuhisa Abo, Akira Yamane, Yoshinobu Asada] The present study investigated the function of miR-1 and miR-133a during the postnatal development of mouse skeletal muscles. The amounts of miR-1 and miR-133a were measured in mouse masseter and gastrocnemius muscles between 1 and 12weeks after birth with real-time polymerase chain reaction and those of HDACs, MEF2, MyoD family, MCK, SRF, and Cyclin D1 were measured at 2 and 12weeks with Western blotting. In both the masseter and gastrocnemius muscles, the amount of miR-1 increased between 1 and 12weeks, whereas the amount of HADC4 decreased between 2 and 12weeks. In the masseter muscle, those of MEF2, MyoD, Myogenin, and MCK increased between 2 and 12weeks, whereas, in the gastrocnemius muscle, only those of MRF4 and MCK increased. The extent of these changes in the masseter muscle was greater than that in the gastrocnemius muscle. The amounts of miR-133a, SRF, and Cyclin D1 did not change significantly in the masseter muscle between 1 and 12weeks after birth. By contrast, in the gastrocnemius muscle, the amounts of miR-133a and Cyclin D1 increased, whereas that of SRF decreased. Our findings suggest that the regulatory pathway of miR-1 via HDAC4 and MEF2 plays a more prominent role during postnatal development in the masseter muscle than in the gastrocnemius muscle, whereas that of miR-133a via SRF plays a more prominent role in the gastrocnemius muscle than in the masseter muscle. Springer Science+Business Media New York, 2015 miRNA nationallicence HDAC4 nationallicence MEF2 nationallicence SRF nationallicence Skeletal muscles nationallicence Nariyama Megumi Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Mori Manami Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Shimazaki Emi Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Ando Hitoshi Department of Biophysics, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Ohnuki Yoshiki Department of Physiology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Abo Tokuhisa Department of Biophysics, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Yamane Akira Department of Biophysics, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Asada Yoshinobu Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 17-27 0300-8177 407:1-2<17 2015 407 11010 https://doi.org/10.1007/s11010-015-2450-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2450-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nariyama Megumi Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Mori Manami Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Shimazaki Emi Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Ando Hitoshi Department of Biophysics, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Ohnuki Yoshiki Department of Physiology, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Abo Tokuhisa Department of Biophysics, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Yamane Akira Department of Biophysics, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 700 1- Asada Yoshinobu Department of Pediatric Dentistry, Tsurumi University School of Dental Medicine, 2-1-3 Tsurumi, Tsurumi-ku, 230-8501, Yokohama, Japan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 17-27 0300-8177 407:1-2<17 2015 407 11010