caa a22 4500 606235590 CHVBK 20210128101243.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2472-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2472-5 Estrogen deprivation does not affect vascular heat shock response in female rats: a comparison with oxidative stress markers [Elektronische Daten] [Antônio Miragem, Mirna Ludwig, Thiago Heck, Fernanda Baldissera, Analu dos Santos, Matias Frizzo, Paulo Homem de Bittencourt Jr.] Hot flashes, which involve a tiny rise in core temperature, are the most common complaint of peri- and post-menopausal women, being tightly related to decrease in estrogen levels. On the other hand, estradiol (E2) induces the expression of HSP72, a member of the 70kDa family of heat shock proteins (HSP70), which are cytoprotective, cardioprotective, and heat inducible. Since HSP70 expression is compromised in age-related inflammatory diseases, we argued whether the capacity of triggering a robust heat shock (HS) response would be still present after E2 withdrawal. Hence, we studied the effects of HS treatment (hot tub) in female Wistar rats subjected to bilateral ovariectomy (OVX) after a 7-day washout period. Twelveh after HS, the animals were killed and aortic arches were surgically excised for molecular analyses. The results were compared with oxidative stress markers in the plasma (superoxide dismutase, catalase, and lipoperoxidation) because HSP70 expression is also sensitive to redox regulation. Extracellular (plasma) to intracellular HSP70 ratio, an index of systemic inflammatory status, was also investigated. The results showed that HS response was preserved in OVX animals, as inferred from HSP70 expression (up to 40% rise, p<0.01) in the aortas, which was accompanied by no further alterations in oxidative stress, hematological parameters, and glycemic control either. This suggests that the lack of estrogen per se could not be solely ascribed as the unique source of low HSP70 expression as observed in long-term post-menopausal individuals. As a consequence, periodic evaluation of HSP70 status (iHSP70 vs. eHSP70) may be of clinical relevance because decreased HS response capacity is at the center of the onset of menopause-related dysfunctions. Springer Science+Business Media New York, 2015 Heat shock proteins nationallicence HSP70 nationallicence Estrogen deprivation nationallicence Menopause nationallicence Stress response nationallicence HS : Heat shock nationallicence HSP70 : The 70kDa family of heat shock proteins nationallicence E2 : Estradiol nationallicence Miragem Antônio Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite 500, ICBS, 2° andar, 90050-170, Porto Alegre, RS, Brazil aut Ludwig Mirna Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut Heck Thiago Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut Baldissera Fernanda Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut dos Santos Analu Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut Frizzo Matias Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut Homem de Bittencourt Jr. Paulo Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite 500, ICBS, 2° andar, 90050-170, Porto Alegre, RS, Brazil aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 239-249 0300-8177 407:1-2<239 2015 407 11010 https://doi.org/10.1007/s11010-015-2472-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2472-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miragem Antônio Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite 500, ICBS, 2° andar, 90050-170, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Ludwig Mirna Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut NATIONALLICENCE 700 1- Heck Thiago Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut NATIONALLICENCE 700 1- Baldissera Fernanda Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut NATIONALLICENCE 700 1- dos Santos Analu Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut NATIONALLICENCE 700 1- Frizzo Matias Department of Life Sciences, Regional University of the Northwest of Rio Grande do Sul State, Ijuí, RS, Brazil aut NATIONALLICENCE 700 1- Homem de Bittencourt Jr Paulo Laboratory of Cellular Physiology, Department of Physiology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Rua Sarmento Leite 500, ICBS, 2° andar, 90050-170, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 239-249 0300-8177 407:1-2<239 2015 407 11010