caa a22 4500 606235639 CHVBK 20210128101243.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2460-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2460-9 The collective nuclear migration of p53 and phosphorylated S473 of Akt during ellipticine-mediated apoptosis in human lung epithelial cancer cells [Elektronische Daten] [Jing-Ping Wang, Ya-Chu Yu, Shih-Ping Chen, Huan-Chang Liang, Chia-Wei Lin, Kang Fang] Topoisomerase II inhibitor ellipticine effectively suppressed the growth of human non-small-cell-lung-cancer (NSCLC) epithelial cells. Previously, we reported the drug activity was consummated through parallel nucleus migration of p53 and Akt in A549 cells. While inducing cell death, the drug activity was proved related to autophagy through phosphorylated Akt at S473. In addition, ellipticine induced cytotoxicity in p53-null H1299 cells with stable expression of ectopic p53. In this work, we further demonstrated that dominant-negative Akt S473A or p53 shRNA inhibited ellipticine-mediated translocalization of p53 and Akt and attenuated apoptotic cell death in A549 cells. The presence of p53 predates ellipticine-mediated apoptotic cell death,assists in nucleus translocation of phosphorylated Akt and activation of autophagy pathway. Growth inhibition through collaborating p53 and phosphorylated Akt473 in lung epithelial cancer cells provided a new perspective of the topoisomerase inhibitor as an effective cancer therapy agent. Springer Science+Business Media New York, 2015 Ellipticine nationallicence Akt nationallicence p53 nationallicence Human non-small-cell-lung-cancer cells nationallicence Apoptosis nationallicence Wang Jing-Ping Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut Yu Ya-Chu Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut Chen Shih-Ping Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut Liang Huan-Chang Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut Lin Chia-Wei Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut Fang Kang Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 123-133 0300-8177 407:1-2<123 2015 407 11010 https://doi.org/10.1007/s11010-015-2460-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2460-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Jing-Ping Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Yu Ya-Chu Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Chen Shih-Ping Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Liang Huan-Chang Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Lin Chia-Wei Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut NATIONALLICENCE 700 1- Fang Kang Department of Life Science, National Taiwan Normal University, Taipei, Taiwan aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 123-133 0300-8177 407:1-2<123 2015 407 11010