caa a22 4500 606235647 CHVBK 20210128101243.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2448-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2448-5 Arginase I expression is upregulated by dietary restriction in the liver of mice as a function of age [Elektronische Daten] [Teikur Majaw, Ramesh Sharma] Arginase is a cytosolic enzyme that catalyzes the hydrolysis of l-arginine to l-ornithine and urea. This reaction comprises the final step of the urea cycle, which provides the principal route for the disposal of nitrogenous waste from protein catabolism. The present study investigates the normal endogenous activity and expression level of arginase I as a function of age in the liver of 2-, 6-, and 18-month-old mice. The effect of dietary restriction (DR) on the expression of arginase I was also investigated in two age groups of mice, 2- and 18-month old. Arginase I activity was assessed spectrophotometrically, and the level of arginase I protein was further confirmed by Western blotting analyses. Arginase I mRNA level was measured using real-time PCR. Our results show that the arginase I activity (U/mg protein) and protein level in liver was higher in 2-month-old mice and decreased gradually with age. In contrast, arginase I mRNA was observed to be higher in the older mice as compared to the younger mice. DR was seen to upregulate the arginase I activity and expression in both 2- and 18-month-old mice. The findings concluded that arginase I is down-regulated with the advancement of age in the liver of mice and is upregulated by DR. This suggests that DR plays an important role in maintaining related metabolic processes as a function of age in mice. Springer Science+Business Media New York, 2015 Arginase I nationallicence Dietary restriction nationallicence Liver nationallicence Mice nationallicence Aging nationallicence Majaw Teikur Department of Biochemistry, North-Eastern Hill University, 793 022, Shillong, India aut Sharma Ramesh Department of Biochemistry, North-Eastern Hill University, 793 022, Shillong, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 1-7 0300-8177 407:1-2<1 2015 407 11010 https://doi.org/10.1007/s11010-015-2448-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2448-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Majaw Teikur Department of Biochemistry, North-Eastern Hill University, 793 022, Shillong, India aut NATIONALLICENCE 700 1- Sharma Ramesh Department of Biochemistry, North-Eastern Hill University, 793 022, Shillong, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 1-7 0300-8177 407:1-2<1 2015 407 11010