caa a22 4500 606235701 CHVBK 20210128101244.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2451-x doi (NATIONALLICENCE)springer-10.1007/s11010-015-2451-x Response of endothelial cells and pericytes to hypoxia and erythropoietin in a co-culture assay dedicated to soft tissue repair [Elektronische Daten] [Gerrit Schneider, Monika Bubel, Tim Pohlemann, Martin Oberringer] The increasing mean life expectancy of the citizens of the western world countries leads to an increase of the age-related diseases, among them soft tissue defects exhibiting inadequate healing. In order to develop new therapeutic strategies to support disturbed soft tissue repair, there is a strong need of sophisticated in vitro assays. A new assay combining scratch wounding with co-cultures of primary human microvascular endothelial cells (HDMEC) and pericytes (HPC) focuses on basic characteristics of cell interaction against the background of soft tissue repair. The cell parameters proliferation, migration and differentiation, and the release of monocyte chemoattractant protein-1 (MCP-1) were analysed in response to hypoxia (pO2<5mmHg) and to erythropoietin (EPO; 50IU/ml), a glycoprotein hormone having shown promising effects in soft tissue repair. As basic characteristics of the assay, direct cell contact in co-culture led to a weakened proliferation of both cell types, an increase of the percentage of myofibroblast-like pericytes and to a higher release of MCP-1. Hypoxia caused a proliferation decrease of HPC in co-culture, which was slightly attenuated by EPO. Hypoxia also reduced the MCP-1 release of co-cultured cells, when EPO had been added. In addition, EPO had a rather positive effect on HPC migration under hypoxia. These in vitro results allow new insights into the interaction of pericytes with endothelial cells in the context of soft tissue repair. Springer Science+Business Media New York, 2015 Wound healing nationallicence Soft tissue repair nationallicence Pericyte nationallicence Endothelial cell nationallicence Hypoxia nationallicence Erythropoietin nationallicence Schneider Gerrit Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut Bubel Monika Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut Pohlemann Tim Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut Oberringer Martin Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 29-40 0300-8177 407:1-2<29 2015 407 11010 https://doi.org/10.1007/s11010-015-2451-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2451-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schneider Gerrit Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut NATIONALLICENCE 700 1- Bubel Monika Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut NATIONALLICENCE 700 1- Pohlemann Tim Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut NATIONALLICENCE 700 1- Oberringer Martin Department of Trauma-, Hand- and Reconstructive Surgery, Saarland University, Kirrberger Strasse, Bldng. 57, 66421, Homburg, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 29-40 0300-8177 407:1-2<29 2015 407 11010