caa a22 4500 606235728 CHVBK 20210128101244.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2463-6 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2463-6 Up-regulation of IL-12 expression in patients with chronic hepatitis B is mediated by the PI3K/Akt pathway [Elektronische Daten] [Hong-wei Wang, Hai-li Gao, Xiao-xia Wei, Xin-hong Wang] Hepatitis B virus (HBV) replicates noncytopathically in hepatocytes, but HBV or proteins encoded by HBV genome could induce cytokines, chemokines expression by hepatocytes.IL-12 is a typical proinflammatory cytokine that plays a critical role in host defense against pathogens, including the HBV. However, the role of IL-12 in chronic hepatitis B (CHB) remains unclear. The aims of this study were to detect the expression of IL-12 in CHB patients and explore the molecular mechanism of HBV-induced IL-12 expression. The results showed that serum levels and hepatic expression of IL-12 were significantly upregulated in CHB patients. HBx protein increased IL-12 expression in a dose-dependent manner. Furthermore, inhibition of PI3K/Akt significantly decreased the HBx-induced IL-12 expression and Akt activation. Taken together, these results indicate that the molecular mechanism of HBV-induced IL-12 expression involves activation of the PI3K/Akt pathway by HBx, leading to transactivation of the IL-12 p35 and p40 promoters. Springer Science+Business Media New York, 2015 Interleukin-12 (IL-12) nationallicence Chronic hepatitis B (CHB) nationallicence Hepatitis B virus (HBV) nationallicence PI3K/Akt pathway nationallicence Wang Hong-wei Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut Gao Hai-li Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut Wei Xiao-xia Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut Wang Xin-hong Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 135-142 0300-8177 407:1-2<135 2015 407 11010 https://doi.org/10.1007/s11010-015-2463-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2463-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Hong-wei Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut NATIONALLICENCE 700 1- Gao Hai-li Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut NATIONALLICENCE 700 1- Wei Xiao-xia Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut NATIONALLICENCE 700 1- Wang Xin-hong Department of Infection Diseases, The First Affiliated Hospital of Xinxiang Medical University, 453100, Weihui, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 135-142 0300-8177 407:1-2<135 2015 407 11010