caa a22 4500 606235736 CHVBK 20210128101244.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s11010-015-2470-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2470-7 Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development [Elektronische Daten] [Mikella Daigle, Pauline Roumaud, Luc Martin] SRY-related box (Sox) transcription factors are conserved among vertebrate species. These proteins regulate multiple processes including sex determination and testis differentiation of the male embryo. Although members of the Sox family have been identified in pre- and postnatal Sertoli cells, they have never been characterized in adult Leydig cells. The objectives of this research were to identify expressions of Sox9, Sox5, and Sox13 in mice Leydig cell cultures and to establish their expression profiles in postnatal mice testes at different developmental stages. Methods used include Western blots and qPCR of stimulated MA-10 cell cultures and whole mice testes. Sox9, Sox5, and Sox13 proteins were detected in MA-10 cells as well as whole mouse testis. Although Sox9, Sox5, and Sox13 mRNA levels from whole mice testes tended to increase according to postnatal development, these results were not significant. Sox members were also detected in whole mice testis by Western Blot. However, Sox9, Sox5, and Sox13 protein expressions remained relatively constant during postnatal development from postnatal (P) day 60 to P365. Being newly characterized in the mouse testis, Sox13 was mainly localized by immunofluorescence within the nuclei of cells from seminiferous tubules, possibly spermatocytes and Sertoli cells. In addition, Sox9, Sox5, and Sox13 proteins were characterized in the nuclei of MA-10 Leydig cell cultures. Their expressions and transcriptional activities remained unaffected by activators of the cAMP/PKA pathway. Thus, Sox9, Sox5, and Sox13 transcription factors are expressed in postnatal testis and may regulate multiple functions such as steroidogenesis and spermatogenesis. Springer Science+Business Media New York, 2015 Sox9 nationallicence Sox5 nationallicence Sox13 nationallicence Puberty nationallicence Leydig cells nationallicence Seminiferous tubules nationallicence Steroidogenesis nationallicence Daigle Mikella Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, E1A 3E9, Moncton, NB, Canada aut Roumaud Pauline Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, E1A 3E9, Moncton, NB, Canada aut Martin Luc Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, E1A 3E9, Moncton, NB, Canada aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 209-221 0300-8177 407:1-2<209 2015 407 11010 https://doi.org/10.1007/s11010-015-2470-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2470-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Daigle Mikella Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, E1A 3E9, Moncton, NB, Canada aut NATIONALLICENCE 700 1- Roumaud Pauline Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, E1A 3E9, Moncton, NB, Canada aut NATIONALLICENCE 700 1- Martin Luc Department of Biology, Université de Moncton, 18, Avenue Antonine Maillet, E1A 3E9, Moncton, NB, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 407/1-2(2015-09-01), 209-221 0300-8177 407:1-2<209 2015 407 11010