caa a22 4500 606235809 CHVBK 20210128101244.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2558-0 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2558-0 Widdrol-induced lipolysis is mediated by PKC and MEK/ERK in 3T3-L1 adipocytes [Elektronische Daten] [Hyun Jeong, Hee Yun, Byung Kim, Eun Lee, Hyun Kwon] Obesity is a serious medical condition causing various diseases such as heart disease, type-2 diabetes, and cancer. Fat cells (adipocytes) play an important role in the generation of energy through hydrolysis of lipids they accumulate. Therefore, induction of lipolysis (breakdown of lipids into fatty acids and glycerol), is one of the ways to treat obesity. In the present study, we investigated the lipolytic effect of widdrol in 3T3-L1 adipocytes and its mechanism. Widdrol considerably increased the amount of glycerol released from 3T3-L1 adipocytes into the medium in a time- and dose-dependent manner. To determine the mechanism of this effect, we investigated the alterations in glycerol release and protein expression in 3T3-L1 adipocytes treated with widdrol alone or widdrol and inhibitors of proteins involved in the cAMP-dependent pathway or cAMP-independent PKC-MAPK pathway, which are known to induce lipolysis in adipocytes. The adenylyl cyclase inhibitor SQ-22536, PLA2 inhibitor dexamethasone, PI3K inhibitor wortmannin, and PKA inhibitor H-89, which were used to investigate the involvement of the cAMP-dependent pathway, did not affect the lipolytic effect of widdrol. Widdrol-induced phosphorylation of PKC, MEK, and ERK, which are related to the PKC-MAPK pathway, and their phosphorylation was inhibited by their inhibitors (H-7, U0126, and PD-98059, respectively). Moreover, the increase in glycerol release induced by widdrol was almost completely blocked by PKC, MEK, and ERK inhibitors. These results suggest that widdrol induces lipolysis through activation of the PKC-MEK-ERK pathway. Springer Science+Business Media New York, 2015 Glycerol release nationallicence Lipolysis nationallicence Widdrol nationallicence Protein kinase C nationallicence Mitogen-activated protein kinase nationallicence Jeong Hyun Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut Yun Hee Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut Kim Byung Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut Lee Eun Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut Kwon Hyun Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 247-254 0300-8177 410:1-2<247 2015 410 11010 https://doi.org/10.1007/s11010-015-2558-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2558-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jeong Hyun Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Yun Hee Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kim Byung Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Lee Eun Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut NATIONALLICENCE 700 1- Kwon Hyun Blue-Bio Industry RIC, Dong-Eui University, 614-714, Busan, Republic of Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 247-254 0300-8177 410:1-2<247 2015 410 11010