caa a22 4500 606235833 CHVBK 20210128101244.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2557-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2557-1 Gensenoside Rb1 protects rat PC12 cells from oxidative stress-induced endoplasmic reticulum stress: the involvement of thioredoxin-1 [Elektronische Daten] [Xian-Si Zeng, Jin-Jing Jia, Li-Fang Ma] Oxidative stress plays an important role in many diseases and hydrogen peroxide (H2O2) plays a central role in the stress. Gensenoside Rb1 is the one of active ingredients in the traditional Chinese medicine Panax notoginseng. It has been reported that gensenoside Rb1 possesses various pharmacological activities. Here we report thatgensenoside Rb1exhibits potent protective effects againstoxidativeinjury induced by H2O2 through inhibiting endoplasmic reticulum stress in PC12 cells. Cell viability assay demonstrated that incubation with H2O2 for 24h led to a significant loss of cultured rat PC12 cells, and the cell viability was pronouncedly increased by pretreatment ofgensenoside Rb1 for 24h. H2O2-induced endoplasmic reticulum stress pathway was also suppressed after gensenoside Rb1 pretreatment, which was related with thioredoxin-1 (Trx-1) induction. Trx-1 siRNA abolished the protective effects of gensenoside Rb1. Our results of the present study demonstrate thatgensenoside Rb1shows a potent anti-oxidativeeffect on cultured PC12 cells by inducing Trx-1 expression. Springer Science+Business Media New York, 2015 Gensenoside Rb1 nationallicence Oxidative stress nationallicence Endoplasmic reticulum stress nationallicence Thioredoxin-1 nationallicence Cdc37 : Cell division cycle 37 nationallicence CHOP : C/EBP homologous protein nationallicence GRP78 : Glucose-regulated protein 78 nationallicence H2O2 : Hydrogen peroxide nationallicence Hsp90 : Heat shock protein 90 nationallicence IRE1 : Inositol-requiring enzyme 1 nationallicence NF-κB : Nuclear factor kappaB nationallicence Nrf2 : Nuclear factor erythroid 2-related factor 2 nationallicence PD : Parkinson disease nationallicence PNS : Panax notogensenoside nationallicence ROS : Reactive oxygen species nationallicence Trx-1 : Thioredoxin-1 nationallicence Zeng Xian-Si College of Life Science, Xinyang Normal University, 464000, Xinyang, China aut Jia Jin-Jing College of Life Science, Xinyang Normal University, 464000, Xinyang, China aut Ma Li-Fang College of Life Science, Xinyang Normal University, 464000, Xinyang, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 239-246 0300-8177 410:1-2<239 2015 410 11010 https://doi.org/10.1007/s11010-015-2557-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2557-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng Xian-Si College of Life Science, Xinyang Normal University, 464000, Xinyang, China aut NATIONALLICENCE 700 1- Jia Jin-Jing College of Life Science, Xinyang Normal University, 464000, Xinyang, China aut NATIONALLICENCE 700 1- Ma Li-Fang College of Life Science, Xinyang Normal University, 464000, Xinyang, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 239-246 0300-8177 410:1-2<239 2015 410 11010