caa a22 4500 60623585X CHVBK 20210128101244.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2539-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2539-3 Cross-talk between macrophages, smooth muscle cells, and endothelial cells in response to cigarette smoke: the effects on MMP2 and 9 [Elektronische Daten] [Abhijit Ghosh, L. Pechota, Gilbert Upchurch Jr., Jonathan Eliason] We hypothesized that matrix metalloproteinase secretion in response to cigarette smoke is modulated by cross-talk between resident cells within the aorta, namely, aortic smooth muscles, endothelial cells, and infiltrating macrophages, and this may be crucial for in vivo formation/progression of abdominal aortic aneurysm (AAA). Cigarette smoke extract (CSE) was applied to rat aortic smooth muscle (RASMC), endothelial (RAEC) or RAW cells, and conditioned media (CSE-CM) collected. Fresh cells were treated with CSE-CM for 24h and then maintained in serum-free medium (SFM) for 72h to analyze MMP2 and MMP9 in media by zymography and the ratio (pS/pJ) of phospho-Stat3 (pStat3) and phospho-Jak2 (pJak2) inside the cells by Western blot. We observed that CSE-CM from RAW and RAEC increased MMP9 by 200 and 17%, respectively, in RASMC and also increased pS/pJ ratio (305 and 228%, respectively) in RASMC. RAW cell-derived CSE-CM induced RAEC to produce moderate amounts of MMP2 (17%), MMP9 (30%), and a 137% increase in pS/pJ. RAW cells receiving unstimulated CM from RASMC and RAEC produced significant amounts of MMP9 (128 and 155%, respectively) and increased pS/pJ (45 and 1283%, respectively). CSE-CM from RASMC and RAEC induced significant production of MMP9 from RAW cells (237 and 162%, respectively) and increase in pS/pJ ratios (1348 and 1494%, respectively). This is the first in vitro study demonstrating cigarette smoke extract-mediated differential interactions between resident cells in the aorta leads to altered modulation of signaling molecules that may be vital for AAA formation under in vivo conditions. Springer Science+Business Media New York, 2015 Abdominal aortic aneurysm nationallicence Cigarette smoke nationallicence MMP2 nationallicence MMP9 nationallicence Cellular interactions nationallicence Ghosh Abhijit Section of Vascular Surgery, Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan Medical School, Ann Arbor, MI, USA aut Pechota L. Section of Vascular Surgery, Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan Medical School, Ann Arbor, MI, USA aut Upchurch Jr. Gilbert Division of Vascular and Endovascular Surgery, University of Virginia, PO Box 800679, Charlottesville, VA, USA aut Eliason Jonathan Section of Vascular Surgery, Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan Medical School, Ann Arbor, MI, USA aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 75-84 0300-8177 410:1-2<75 2015 410 11010 https://doi.org/10.1007/s11010-015-2539-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2539-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ghosh Abhijit Section of Vascular Surgery, Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan Medical School, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Pechota L. Section of Vascular Surgery, Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan Medical School, Ann Arbor, MI, USA aut NATIONALLICENCE 700 1- Upchurch Jr Gilbert Division of Vascular and Endovascular Surgery, University of Virginia, PO Box 800679, Charlottesville, VA, USA aut NATIONALLICENCE 700 1- Eliason Jonathan Section of Vascular Surgery, Department of Surgery, Jobst Vascular Research Laboratories, University of Michigan Medical School, Ann Arbor, MI, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 75-84 0300-8177 410:1-2<75 2015 410 11010