caa a22 4500 606235876 CHVBK 20210128101244.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2547-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2547-3 Identification and profiling of miRNAs in the freeze-avoiding gall moth Epiblema scudderiana via next-generation sequencing [Elektronische Daten] [Pierre Lyons, Nicolas Crapoulet, Kenneth Storey, Pier Morin] The rapid development of high-throughput next-generation sequencing approaches in recent years has facilitated large-scale discovery and expression analysis of non-coding RNAs, including miRNAs, in traditional and non-traditional animal models. Such an approach has been leveraged to amplify, identify, and quantify miRNAs in several models of cold adaptation. The present study is the first to investigate the status of these small RNAs in an insect species that uses the freeze avoidance strategy of cold hardiness, the gall moth Epiblema scudderiana. To characterize the overall miRNA expression profile and to identify cold-modulated miRNAs in control (5°C) and cold-exposed (−15°C) E. scudderiana larvae, a next-generation sequencing-based approach was undertaken. A total of 44 differentially expressed miRNAs were identified between the two conditions; 21 up-regulated miRNAs and 23 down-regulated miRNAs in −15°C-exposed larvae as compared with controls. Among the most significant changes observed in miRNAs with potential relevance to cold adaptation were elevated miR-1-3p, miR-92b-3p, and miR-133-3p levels as well as reduced miR-13a-3p and miR-13b-3p levels in E. scudderiana larvae exposed to cold temperatures. Expression values obtained from next-generation sequencing were also validated by a quantitative PCR approach for five miRNAs; miR-34-5p, miR-274-5p, miR-275-3p, miR-307a-3p, and miR-316-5p. Overall, this work provides the first description of a miRNA signature for subzero survival by a freeze-avoiding insect using a high-throughput approach and positions a new group of miRNAs at the forefront of the molecular changes underlying cold adaptation. Springer Science+Business Media New York, 2015 Cold hardiness nationallicence Freeze avoidance nationallicence Hypometabolism nationallicence MicroRNAs nationallicence Translational regulation nationallicence Lyons Pierre Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, E1A 3E9, Moncton, NB, Canada aut Crapoulet Nicolas Atlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, E1C 8X3, Moncton, NB, Canada aut Storey Kenneth Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut Morin Pier Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, E1A 3E9, Moncton, NB, Canada aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 155-163 0300-8177 410:1-2<155 2015 410 11010 https://doi.org/10.1007/s11010-015-2547-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2547-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lyons Pierre Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, E1A 3E9, Moncton, NB, Canada aut NATIONALLICENCE 700 1- Crapoulet Nicolas Atlantic Cancer Research Institute, Pavillon Hôtel-Dieu 35 Providence Street, E1C 8X3, Moncton, NB, Canada aut NATIONALLICENCE 700 1- Storey Kenneth Institute of Biochemistry, Carleton University, 1125 Colonel By Drive, K1S 5B6, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Morin Pier Department of Chemistry and Biochemistry, Université de Moncton, 18 Antonine-Maillet Avenue, E1A 3E9, Moncton, NB, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 155-163 0300-8177 410:1-2<155 2015 410 11010