caa a22 4500 606235884 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2549-1 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2549-1 Cardiolipin composition correlates with prostate cancer cell proliferation [Elektronische Daten] [Anja Sapandowski, Matthias Stope, Katja Evert, Matthias Evert, Uwe Zimmermann, Daniela Peter, Ilona Päge, Martin Burchardt, Lorenz Schild] Prostate cancer (PC) is the second most diagnosed cancer in men. It has been recognized that diet can play a crucial role in PC genesis and progression. In this context, free fatty acids are considered as modulators of cell proliferation. Recently, a relationship between the composition of the mitochondrial phospholipid cardiolipin (CL) and cell proliferation has been discussed. The aim of this study was to analyse the interrelationship between CL composition and the proliferation of prostate cells by exposing PC-3 tumour cells to different fatty acids and by analysing the CL composition in prostate tissue from PC patients after prostatectomy. Among the applied fatty acids, palmitic acid was found to stimulate proliferation of PC-3 cells, whereas oleic acid (OA) had an inhibiting effect. The lipidomic analysis of CL revealed that fatty acids supplied to PC-3 cells were incorporated into CL molecules. Further, the CL content of palmitoleic acid (C16:1) exclusively correlated with the proliferation of PC-3 cells. The CL composition significantly differed between tumour and normal prostate tissue from PC patients. In five out of six patients, the CL content of palmitoleic acid was higher in tumour prostate tissue in comparison to normal prostate tissue. Our data illustrate that the composition of CL can be easily modified by the fatty acid environment of cells. OA was most effective in decreasing the amount of palmitoleic acid within the CL molecules and deceleration of PC-3 cell proliferation. In conclusion, a diet rich in OA might be beneficial in protecting from rapid proliferation of PC cells. Springer Science+Business Media New York, 2015 Prostate cancer nationallicence Free fatty acids nationallicence Cardiolipin nationallicence Lipidomic analysis nationallicence Tumour cell proliferation nationallicence PC : Prostate cancer nationallicence CL : Cardiolipin nationallicence LA : Linoleic acid nationallicence SA : Stearic acid nationallicence AA : Arachidonic acid nationallicence DA : Docosahexaenoic acid nationallicence PA : Palmitic acid nationallicence OA : Oleic acid nationallicence ME : Methanol nationallicence MTT : 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide nationallicence Sapandowski Anja Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Stope Matthias Department for Urology, University Medicine Greifswald, Greifswald, Germany aut Evert Katja Department for Pathology, University Medicine Greifswald, Greifswald, Germany aut Evert Matthias Department for Pathology, University Medicine Greifswald, Greifswald, Germany aut Zimmermann Uwe Department for Urology, University Medicine Greifswald, Greifswald, Germany aut Peter Daniela Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Päge Ilona Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Burchardt Martin Department for Urology, University Medicine Greifswald, Greifswald, Germany aut Schild Lorenz Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 175-185 0300-8177 410:1-2<175 2015 410 11010 https://doi.org/10.1007/s11010-015-2549-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2549-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sapandowski Anja Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Stope Matthias Department for Urology, University Medicine Greifswald, Greifswald, Germany aut NATIONALLICENCE 700 1- Evert Katja Department for Pathology, University Medicine Greifswald, Greifswald, Germany aut NATIONALLICENCE 700 1- Evert Matthias Department for Pathology, University Medicine Greifswald, Greifswald, Germany aut NATIONALLICENCE 700 1- Zimmermann Uwe Department for Urology, University Medicine Greifswald, Greifswald, Germany aut NATIONALLICENCE 700 1- Peter Daniela Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Päge Ilona Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 700 1- Burchardt Martin Department for Urology, University Medicine Greifswald, Greifswald, Germany aut NATIONALLICENCE 700 1- Schild Lorenz Department for Clinical Chemistry and Pathological Biochemistry, Otto-von-Guericke University Magdeburg, Leipziger Str. 44, 39120, Magdeburg, Germany aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 175-185 0300-8177 410:1-2<175 2015 410 11010