caa a22 4500 606235892 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2543-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2543-7 Epigenetic regulation of human adipose-derived stem cells differentiation [Elektronische Daten] [Kristina Daniunaite, Inga Serenaite, Roberta Misgirdaite, Juozas Gordevicius, Ausra Unguryte, Sandrine Fleury-Cappellesso, Eiva Bernotiene, Sonata Jarmalaite] Adult stem cells have more restricted differentiation potential than embryonic stem cells (ESCs), but upon appropriate stimulation can differentiate into cells of different germ layers. Epigenetic factors, including DNA modifications, take a significant part in regulation of pluripotency and differentiation of ESCs. Less is known about the epigenetic regulation of these processes in adult stem cells. Gene expression profile and location of DNA modifications in adipose-derived stem cells (ADSCs) and their osteogenically differentiated lineages were analyzed using Agilent microarrays. Methylation-specific PCR and restriction-based quantitative PCR were applied for 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) detection in selected loci. The level of DNA modifications in the POU5F1 locus was quantified with deep sequencing. Expression levels of selected genes were assayed by real-time PCR. ADSCs differentiation into osteogenic lineages involved marked changes in both 5mC and 5hmC profiles, but 5hmC changes were more abundant. 5mC losses and 5hmC gains were the main events observed during ADSCs differentiation, and were accompanied by increased expression of TET1 (P=0.009). In ADSCs, POU5F1 was better expressed than NANOG or SOX2 (P≤0.001). Both 5mC and 5hmC marks were present in the POU5F1 locus, but only hydroxymethylation of specific cytosine showed significant effect on the gene expression. In summary, the data of our study suggest significant involvement of changes in 5hmC profile during the differentiation of human adult stem cells. Springer Science+Business Media New York, 2015 Adipose-derived stem cells nationallicence 5-hydroxymethylcytosine nationallicence POU5F1 nationallicence Daniunaite Kristina Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut Serenaite Inga Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut Misgirdaite Roberta Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut Gordevicius Juozas Institute of Mathematics and Informatics, Vilnius University, Akademijos 4, 08663, Vilnius, Lithuania aut Unguryte Ausra State Research Institute Center of Innovative Medicine, Zygimantu 9, 01120, Vilnius, Lithuania aut Fleury-Cappellesso Sandrine EFS Pyreenees-Mediterranee, 31027, Toulouse, France aut Bernotiene Eiva State Research Institute Center of Innovative Medicine, Zygimantu 9, 01120, Vilnius, Lithuania aut Jarmalaite Sonata Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 111-120 0300-8177 410:1-2<111 2015 410 11010 https://doi.org/10.1007/s11010-015-2543-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2543-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Daniunaite Kristina Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Serenaite Inga Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Misgirdaite Roberta Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Gordevicius Juozas Institute of Mathematics and Informatics, Vilnius University, Akademijos 4, 08663, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Unguryte Ausra State Research Institute Center of Innovative Medicine, Zygimantu 9, 01120, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Fleury-Cappellesso Sandrine EFS Pyreenees-Mediterranee, 31027, Toulouse, France aut NATIONALLICENCE 700 1- Bernotiene Eiva State Research Institute Center of Innovative Medicine, Zygimantu 9, 01120, Vilnius, Lithuania aut NATIONALLICENCE 700 1- Jarmalaite Sonata Division of Human Genome Research Centre, Faculty of Natural Sciences, Vilnius University, Ciurlionio 21, 03101, Vilnius, Lithuania aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 111-120 0300-8177 410:1-2<111 2015 410 11010