caa a22 4500 606235906 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2537-5 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2537-5 Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling [Elektronische Daten] [No-Joon Song, So-Mi Kwon, Suji Kim, Hyang-Jin Yoon, Cho-Rong Seo, Byunghyun Jang, Seo-Hyuk Chang, Jin-Mo Ku, Jeong-Soo Lee, Ki-Moon Park, Joung-Woo Hong, Geun Kim, Kye Won Park] The identification and examination of potential determinants controlling the progression of cell fate toward osteoblasts can be intriguing subjects. In this study, the effects of sulfuretin, a major compound isolated from Rhus verniciflua Stokes, on osteoblast differentiation were investigated. Treatments of sulfuretin induced alkaline phosphatase (ALP) activity in mesenchymal C3H10T1/2 cells and mineralization in preosteoblast MC3T3-E1 cells. Pro-osteogenic effects of sulfuretin were consistently observed in freshly isolated primary bone marrow cells. In mechanical studies, sulfuretin specifically induced expression of TGF-β target genes, such as SMAD7 and PAI-1, but not other signaling pathway-related genes. Similar to the results of gene expression analysis, reporter assays further demonstrated TGF-β-specific induction by sulfuretin. Furthermore, disruption of TGF-β signaling using treatment with TGF-β-specific inhibitor, SB-431542, and introduction of SMAD2/3 small interfering RNA impaired the effects of sulfuretin in inducing ALP activity and expression of ALP mRNA. Together, these data indicate that the pro-osteogenic effects of sulfuretin are mediated through activation of TGF-β signaling, further supporting the potential of sulfuretin in the prevention of bone-related diseases such as bone fracture and osteoporosis. Springer Science+Business Media New York, 2015 Sulfuretin nationallicence Osteoblast differentiation nationallicence TGF-beta nationallicence C3H10T1/2 cells nationallicence MC3T3-E1 cells nationallicence ALP : Alkaline phosphatase nationallicence OCN : Osteocalcin nationallicence OSX : Osterix nationallicence COL I : Collagen I nationallicence MSC : Mesenchymal stem cells nationallicence TGF-β1 : Transforming growth factor-β1 nationallicence WNT : Wingless-int nationallicence BMP : Bone morphogenetic protein nationallicence siRNA : Small interfering RNA nationallicence MTT : 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide nationallicence DMSO : Dimethylsulfoxide nationallicence PCR : Polymerase chain reaction nationallicence BCIP : 5-Bromo-4-cholor-3-indolyl phosphate nationallicence NBT : Nitroblue tetrazolium nationallicence Song No-Joon Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Kwon So-Mi Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Kim Suji Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Yoon Hyang-Jin Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Seo Cho-Rong Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Jang Byunghyun Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Chang Seo-Hyuk Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Ku Jin-Mo Natural Product Research Team, Gyeonggi Bio-Center, Gyeonggi Institute of Science and Technology Promotion, 443-270, Suwon, Republic of Korea aut Lee Jeong-Soo Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 305-806, Daejeon, Republic of Korea aut Park Ki-Moon Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Hong Joung-Woo Graduate School of East-West Medical Science, Kyung Hee University, 446-701, Yongin, Republic of Korea aut Kim Geun Department of Biomechatronic Engineering, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Park Kye Won Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 55-63 0300-8177 410:1-2<55 2015 410 11010 https://doi.org/10.1007/s11010-015-2537-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2537-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song No-Joon Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Kwon So-Mi Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Kim Suji Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Yoon Hyang-Jin Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Seo Cho-Rong Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Jang Byunghyun Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Chang Seo-Hyuk Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Ku Jin-Mo Natural Product Research Team, Gyeonggi Bio-Center, Gyeonggi Institute of Science and Technology Promotion, 443-270, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Lee Jeong-Soo Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 305-806, Daejeon, Republic of Korea aut NATIONALLICENCE 700 1- Park Ki-Moon Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Hong Joung-Woo Graduate School of East-West Medical Science, Kyung Hee University, 446-701, Yongin, Republic of Korea aut NATIONALLICENCE 700 1- Kim Geun Department of Biomechatronic Engineering, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 700 1- Park Kye Won Department of Food Science and Biotechnology, Sungkyunkwan University, 440-746, Suwon, Republic of Korea aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 55-63 0300-8177 410:1-2<55 2015 410 11010