caa a22 4500 606235914 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2548-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2548-2 Telopodes of telocytes are influenced in vitro by redox conditions and ageing [Elektronische Daten] [Ana-Maria Enciu, Laurentiu Popescu] Telocytes (TCs) are a novel cell type identified among interstitial cells in various organs. TCs are characterized by very long cell processes (tens to hundreds micrometres) named telopodes (Tps) with uneven calibre: dilations (podoms) and very thin segments (podomers). However, little is known about the factors which influence Tps conformation. Recently, extracellular matrix proteins were found to influence Tps extension, adherence and spreading. Here, we show that oxidative stress and ageing influence formation of new Tps of TCs cultivated from human non-pregnant myometrium. Using real-time videomicroscopy, we found that ageing the TCs to passage 21 increased the ratio of Tps/TC number with about 50%, whereas oxidative stress hindered formation of new Tps in both aged and young TCs (passage 7). Under oxidative stress, newly formed cell processes were up to 25% shorter. Migration pathway length was decreased by 30-40% for both young and aged cells in an oxidative stress environment. Contrary, addition of N-acetyl cysteine in cell culture medium shifted TCs morphology to a long and slender profile. In conclusion, we showed that TCs specific morphology in vitro is influenced by oxidative status balance, as well as ageing. Springer Science+Business Media New York, 2015 Telocytes nationallicence Telopodes nationallicence Ageing nationallicence Oxidative stress nationallicence Cell prolongations nationallicence N -acetyl cysteine nationallicence Cell migration nationallicence Enciu Ana-Maria Department of Cellular, Molecular Biology and Histology, "Carol Davila” University of Medicine and Pharmacy, 8 Eroilor Sanitari, 050474, Bucharest 5, Romania aut Popescu Laurentiu Department of Cellular, Molecular Biology and Histology, "Carol Davila” University of Medicine and Pharmacy, 8 Eroilor Sanitari, 050474, Bucharest 5, Romania aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 165-174 0300-8177 410:1-2<165 2015 410 11010 https://doi.org/10.1007/s11010-015-2548-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2548-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Enciu Ana-Maria Department of Cellular, Molecular Biology and Histology, "Carol Davila” University of Medicine and Pharmacy, 8 Eroilor Sanitari, 050474, Bucharest 5, Romania aut NATIONALLICENCE 700 1- Popescu Laurentiu Department of Cellular, Molecular Biology and Histology, "Carol Davila” University of Medicine and Pharmacy, 8 Eroilor Sanitari, 050474, Bucharest 5, Romania aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 165-174 0300-8177 410:1-2<165 2015 410 11010