caa a22 4500 606235922 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2538-4 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2538-4 The expression of RNA helicase DDX5 is transcriptionally upregulated by calcitriol through a vitamin D response element in the proximal promoter in SiHa cervical cells [Elektronische Daten] [Ramiro González-Duarte, Verna Cázares-Ordoñez, Lorenza Díaz, Víctor Ortíz, Fernando Larrea, Euclides Avila] The DEAD box RNA helicase DDX5 is a multifunctional protein involved in the regulatory events of gene expression. Herein, we presented evidence indicating that DDX5 is transcriptionally upregulated by calcitriol, the hormonal form of vitamin D3. In silico analysis revealed the presence of two putative vitamin D response elements (VDREs) in the DDX5 promoter region. Using luciferase reporter assays, we demonstrated that the DDX5 promoter containing these putative VDREs significantly increased the luciferase activity in vitamin D receptor (VDR)-positive SiHa cells upon calcitriol treatment. Electrophoretic mobility shift assays showed the ability of VDR and retinoid X receptor to interact only with the most proximal VDRE, while chromatin immunoprecipitation analysis confirmed the occupancy of this VDRE by the VDR. Finally, we demonstrated that calcitriol significantly increased both DDX5 mRNA and protein in SiHa cells. In summary, this study shows that DDX5 gene is transcriptionally upregulated by calcitriol through a VDRE located in its proximal promoter. Given the importance of DDX5 as a master regulator of differentiation programs, our study suggests that the pro-differentiating properties of calcitriol may be related with the induction of DDX5. Springer Science+Business Media New York, 2015 Vitamin D nationallicence RNA helicase nationallicence Nuclear receptor nationallicence VDR nationallicence Cervical cancer nationallicence González-Duarte Ramiro Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut Cázares-Ordoñez Verna Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut Díaz Lorenza Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut Ortíz Víctor Department of Physiology of Nutrition, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut Larrea Fernando Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut Avila Euclides Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 65-73 0300-8177 410:1-2<65 2015 410 11010 https://doi.org/10.1007/s11010-015-2538-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2538-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- González-Duarte Ramiro Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Cázares-Ordoñez Verna Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Díaz Lorenza Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Ortíz Víctor Department of Physiology of Nutrition, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Larrea Fernando Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Avila Euclides Department of Reproductive Biology, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Vasco de Quiroga # 15, Col. Sección XVI, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 65-73 0300-8177 410:1-2<65 2015 410 11010