caa a22 4500 606235930 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2536-6 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2536-6 Combined therapeutic efficacy of carvacrol and X-radiation against 1,2-dimethyl hydrazine-induced experimental rat colon carcinogenesis [Elektronische Daten] [Sivaranjani Arivalagan, Nisha Thomas, Balaji Chandrasekaran, Vijay Mani, Aktarul Siddique, Thayalan Kuppsamy, Nalini Namasivayam] Colon cancer is one of the most commonly diagnosed cancers, and is a major cause of cancer morbidity and mortality worldwide. The objective of the present study is to evaluate the combined therapeutic efficacy of carvacrol (CVC) and X-radiation against 1,2-dimethylhydrazine-induced colon cancer. Male albino Wistar rats were randomly divided into six groups. Group 1 served as control; group 2 received 40mg/kgb.wt of CVC orally everyday throughout the experimental period (32weeks); groups 3-6 received subcutaneous injections of DMH (20mg/kgb.wt), once a week for the first 15weeks; group 4 received a single dose of X-radiation at the 31st week; group 5 received CVC (40mg/kgb.wt) two days after the last injection of DMH and continued everyday till the end of the experimental period; group 6 received CVC as in group 5 and radiation as in group 4. DMH-treated rats showed increased incidence of aberrant crypt foci (ACF), dysplastic aberrant crypt foci (DACF), mast cell number, argyrophilic nucleolar organizer regions; elevated activities of phase I enzymes, decreased activities of phase II enzymes, decreased mucin content and altered colonic and liver histology as compared to control rats. Though the individual treatments with CVC and X-radiation to DMH-treated rats reversed the above changes, the combined treatment with both CVC and X-radiation showed a marked effect. Our findings emphasize the potential role of combined therapeutic effect of CVC and X-radiation against DMH-induced colon carcinogenesis. Springer Science+Business Media New York, 2015 Colon cancer nationallicence Carvacrol nationallicence X-radiation nationallicence Aberrant crypt foci nationallicence Mast cell nationallicence Mucin nationallicence Arivalagan Sivaranjani Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut Thomas Nisha Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut Chandrasekaran Balaji Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut Mani Vijay Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut Siddique Aktarul Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut Kuppsamy Thayalan Medical Physics Division, Dr. Kamakshi Memorial Hospital, No: 1, Radial Road, Pallikaranai, 600 100, Chennai, Tamil Nadu, India aut Namasivayam Nalini Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 37-54 0300-8177 410:1-2<37 2015 410 11010 https://doi.org/10.1007/s11010-015-2536-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2536-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arivalagan Sivaranjani Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Thomas Nisha Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Chandrasekaran Balaji Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Mani Vijay Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Siddique Aktarul Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Kuppsamy Thayalan Medical Physics Division, Dr. Kamakshi Memorial Hospital, No: 1, Radial Road, Pallikaranai, 600 100, Chennai, Tamil Nadu, India aut NATIONALLICENCE 700 1- Namasivayam Nalini Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalai Nagar, 608 002, Chennai, Tamil Nadu, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 37-54 0300-8177 410:1-2<37 2015 410 11010