caa a22 4500 606235957 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2556-2 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2556-2 Antioxidant action of six Trifolium species in blood platelet experimental system in vitro [Elektronische Daten] [J. Kolodziejczyk-Czepas, P. Nowak, I. Kowalska, A. Stochmal] This study includes a comparative evaluation of antioxidant effects of plant extracts (1.5-50.0μg/ml), derived from six clover (Trifolium) species: T. alexandrinum L., T. fragiferum L., T. hybridum L., T. incarnatum L., T. resupinatum var. majus Boiss., and T. resupinatum var. resupinatum L. Chemical profiles of the extracts contained three or four groups of (poly)phenolic compounds such as phenolic acids, clovamides, isoflavones, and other flavonoids. Antioxidant properties of Trifolium extracts were assessed as the efficacy to reduce oxidative and nitrative damage to blood platelets, exposed to 100μM peroxynitrite-induced oxidative stress in vitro. Antioxidant actions of the examined extracts were determined by the following biomarkers of oxidative stress: thiol groups, 3-nitrotyrosine, lipid hydroperoxides, and thiobarbituric acid-reactive substances(TBARS). Despite the significant differences in the chemical composition (the total phenolic concentrations varied between 11.30 and 52.55mg/g of dry mass) of Trifolium extracts, we observed noticeable protective effects of almost all tested plant preparations. The T. alexandrinum extract, containing the highest concentration of phenols, was the most effective antioxidant among the tested extracts. On the other hand, the T. incarnatum extract, which contained a comparable total phenolic content (49.77mg/g), was less efficient in prevention of tyrosine nitration and generation of TBARS. These findings indicate on the important role of individual phenolic components of the examined clover extracts for the final antioxidative effects. Antioxidative properties of the remaining extracts were noticeably weaker. The Author(s), 2015 Antioxidant nationallicence Blood platelets nationallicence Clover nationallicence Oxidative stress nationallicence Trifolium nationallicence Kolodziejczyk-Czepas J. Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut Nowak P. Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut Kowalska I. Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Pulawy, Poland aut Stochmal A. Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Pulawy, Poland aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 229-237 0300-8177 410:1-2<229 2015 410 11010 https://doi.org/10.1007/s11010-015-2556-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2556-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kolodziejczyk-Czepas J. Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut NATIONALLICENCE 700 1- Nowak P. Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236, Lodz, Poland aut NATIONALLICENCE 700 1- Kowalska I. Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Pulawy, Poland aut NATIONALLICENCE 700 1- Stochmal A. Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, 24-100, Pulawy, Poland aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 229-237 0300-8177 410:1-2<229 2015 410 11010