caa a22 4500 606235973 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2555-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2555-3 Atypical PKC phosphorylates microtubule affinity-regulating kinase 4 in vitro [Elektronische Daten] [Farha Naz, Asimul Islam, Faizan Ahmad, Md. Hassan] MAP/Microtubule affinity-regulating kinase 4 (MARK4), a Ser/Thr protein kinases, is related to the Par-1 (partitioning-defective) gene products, and is the human ortholog of Par-1. MARK4 shows its role in the cell polarity at the time of embryonic development. It is mostly located at the basal regionof cells, providing apico-basal polarity. Here, we made two variants of human Par-1d (MARK4), kinase domain (MARK4-F2), and kinase domain along with 59N-terminal residues (MARK4-F1) and saw their ATPase hydrolysis in the presence of each other. We observed that the activity of one variant was increased in the presence of other. We also demonstrated that both variants were phosphorylated by atypical PKC and their activities were increased in the presence of increasing concentration of atypical protein kinase c (aPKC). The phosphorylation was observed at the serine and threonine residues of MARK4. The interaction of MARK2 and MARK3 with aPKC and their negative regulation by aPKC is already known. This study confirms a functional link between aPKC and MARK4, two central determinants of cell polarity, and it suggests that aPKC may regulate all four members of Par-1 through phosphorylating them in polarized cells. Springer Science+Business Media New York, 2015 Microtubule affinity-regulating kinase nationallicence Cell polarity nationallicence Atypical protein kinase C nationallicence Par-1 nationallicence Microtubule dynamics nationallicence ATP nationallicence Naz Farha Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut Islam Asimul Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut Ahmad Faizan Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut Hassan Md Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 223-228 0300-8177 410:1-2<223 2015 410 11010 https://doi.org/10.1007/s11010-015-2555-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2555-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Naz Farha Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Islam Asimul Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Ahmad Faizan Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 700 1- Hassan Md Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, Jamia Nagar, 110025, New Delhi, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 223-228 0300-8177 410:1-2<223 2015 410 11010