caa a22 4500 606235981 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2535-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2535-7 NF45 overexpression is associated with poor prognosis and enhanced cell proliferation of pancreatic ductal adenocarcinoma [Elektronische Daten] [Chunhua Wan, Chen Gong, Li Ji, Xiaorong Liu, Yayun Wang, Liang Wang, Mengting Shao, Linlin Yang, Shaoqing Fan, Yin Xiao, Xiaotong Wang, Manhua Li, Guoxiong Zhou, Yixin Zhang] NF45, also referred to as nuclear factor of activated T cells, has been reported to promote the progression of multiple cancer types. However, the expression and physiological significance of NF45 in pancreatic ductal adenocarcinoma (PDAC) remain largely elusive. In this study, we investigated the clinical relevance and potential role of NF45 expression in PDAC development. Western blot analysis revealed that NF45 was remarkably upregulated in PDAC tissues, compared with the adjacent non-tumorous ones. In addition, the expression of NF45 in 122 patients with PDAC was evaluated using immunohistochemistry. In this way, we found that NF45 was abundantly expressed in PDAC tissues, and the expression of NF45 was correlated with tumor size (p=0.007), histological differentiation (p=0.033), and TNM stage (p=0.001). Importantly, patients with low levels of NF45 expression exhibited better postoperative prognosis as compared with those with high NF45 expression. Furthermore, using PDAC cell cultures, we found that interference of NF45 expression using siRNA oligos suppressed PDAC cell proliferation and retarded cell cycle progression. Moreover, depletion of NF45 impaired the levels of cellular cyclin E and proliferating cell nuclear antigen (PCNA). Conversely, overexpression of NF45 facilitated the cell growth and accelerated cell cycle progression. Our results establish NF45 as an important indicator of PDAC prognosis with potential utility as a therapeutic target in this lethal disease. Springer Science+Business Media New York, 2015 NF45 nationallicence Pancreatic ductal adenocarcinoma (PDAC) nationallicence Prognosis nationallicence Cell proliferation nationallicence Wan Chunhua Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, 226001, Nantong, Jiangsu, China aut Gong Chen Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Ji Li Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Liu Xiaorong Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut Wang Yayun Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut Wang Liang Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut Shao Mengting Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut Yang Linlin Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut Fan Shaoqing Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Xiao Yin Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Wang Xiaotong Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Li Manhua Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Zhou Guoxiong Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut Zhang Yixin Department of General Surgery, Nantong University Cancer Hospital, 226001, Nantong, Jiangsu, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 25-35 0300-8177 410:1-2<25 2015 410 11010 https://doi.org/10.1007/s11010-015-2535-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2535-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wan Chunhua Department of Nutrition and Food Hygiene, School of Public Health, Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Gong Chen Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Ji Li Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Liu Xiaorong Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Wang Yayun Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Wang Liang Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Shao Mengting Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Yang Linlin Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Fan Shaoqing Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Xiao Yin Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Wang Xiaotong Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Li Manhua Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Zhou Guoxiong Department of Gastroenterology, Affiliated Hospital of Nantong University, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 700 1- Zhang Yixin Department of General Surgery, Nantong University Cancer Hospital, 226001, Nantong, Jiangsu, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 25-35 0300-8177 410:1-2<25 2015 410 11010