caa a22 4500 606236031 CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2544-6 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2544-6 Comparative biochemical responses and antioxidant activities of the rabbit urinary bladder to whole grapes versus resveratrol [Elektronische Daten] [Johdi-Ann Francis, Robert Leggett, Catherine Schuler, Robert Levin] The objective of this study is to compare the antioxidant activity of a whole-grape suspension with the antioxidant activity or pure resveratrol on the effect of hydrogen peroxide (H2O2) on malondialdehyde (MDA) generation, choline acetyltransferase (ChAT) activity, calcium ATPase activity, and sarcoendoplasmic reticular ATPase (SERCA) of the male rabbit urinary bladder. MDA was used as a model for the effect of H2O2 on lipid peroxidation. ChAT, SERCA, and calcium ATPase were evaluated based on their importance in urinary bladder physiology and pathology. Four male rabbit bladders were used. Each bladder was separated into muscle and mucosa, frozen under liquid nitrogen and stored at −80°C for biochemical evaluation. The effect of H2O2 on the enzymes listed above was determined in the presence and absence of either resveratrol or a whole-grape suspension. (1) Resveratrol was significantly more effective than the grape suspension at protecting the bladder muscle and mucosa against peroxidation as quantitated by MDA formation. (2) The grape suspension was significantly more effective at protecting ChAT activity against oxidative stress of the muscle than resveratrol. (3) Neither the grape suspension nor resveratrol were particularly effective at protecting the bladder muscle or mucosa calcium ATPase or SERCA against oxidative stress. (4) ChAT was significantly more sensitive to oxidative stress than either calcium ATPase or SERCA. These data support the idea that the grape suspension protects the mitochondria and nerve terminals to a significantly greater degree than resveratrol which suggests that the activities of the grape suspension are due to the combination of active components found in the grape suspension and not just resveratrol alone. Springer Science+Business Media New York (outside the USA), 2015 Bladder nationallicence Smooth muscle nationallicence Mucosa nationallicence Antioxidants nationallicence Resveratrol nationallicence Grapes nationallicence Francis Johdi-Ann Albany College of Pharmacy and Health Sciences, 12208, Albany, NY, USA aut Leggett Robert Stratton VA Medical Center, 113 Holland Ave., 12208, Albany, NY, USA aut Schuler Catherine Stratton VA Medical Center, 113 Holland Ave., 12208, Albany, NY, USA aut Levin Robert Albany College of Pharmacy and Health Sciences, 12208, Albany, NY, USA aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 121-129 0300-8177 410:1-2<121 2015 410 11010 https://doi.org/10.1007/s11010-015-2544-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2544-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Francis Johdi-Ann Albany College of Pharmacy and Health Sciences, 12208, Albany, NY, USA aut NATIONALLICENCE 700 1- Leggett Robert Stratton VA Medical Center, 113 Holland Ave., 12208, Albany, NY, USA aut NATIONALLICENCE 700 1- Schuler Catherine Stratton VA Medical Center, 113 Holland Ave., 12208, Albany, NY, USA aut NATIONALLICENCE 700 1- Levin Robert Albany College of Pharmacy and Health Sciences, 12208, Albany, NY, USA aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 121-129 0300-8177 410:1-2<121 2015 410 11010