caa a22 4500 60623604X CHVBK 20210128101245.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s11010-015-2533-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2533-9 Overexpression of SYF2 correlates with enhanced cell growth and poor prognosis in human hepatocellular carcinoma [Elektronische Daten] [Shusen Zhang, Weidong Shi, Yuyan Chen, Zhiwei Xu, Jia Zhu, Tingting Zhang, Wei Huang, Runzhou Ni, Cuihua Lu, Xiubing Zhang] SYF2, also known as p29/NTC31/CBPIN, encodes a nuclear protein that interacts with Cyclin D-type binding-protein 1. SYF2has been reported to be involved in pre-mRNA splicing and cell cycle regulation. In the present study, we observed that SYF2 was obviously upregulated in HCC tumor tissues and cell lines, and its level was positively correlated with the tumor grade and Ki-67 expression, as well as poor prognosis of HCC. In vitro, using serum starvation-refeeding experiment, our results suggested that SYF2 was upregulated in proliferating HCC cells, and was positive correlated with the expression of PCNA and Cyclin D1. In addition, depletion of SYF2 decreased PCNA and Cyclin D1 levels. Accordingly, interference of SYF2 resulted in cells cycle arrest at G1/S phase in Huh7 HCC cells. Furthermore, we found that SYF2 might interact with Cyclin D1 and could confer doxorubicin resistance in HCC cells. These findings revealed that SYF2 might play a regulatory role in the proliferation of HCC cells. In summary, SYF2 may be a novel prognostic marker and serve as a potential therapeutic target in HCC. Springer Science+Business Media New York, 2015 SYF2 nationallicence Hepatocellular carcinoma nationallicence Cyclin D1 nationallicence Prognosis nationallicence Cell proliferation nationallicence Zhang Shusen Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Shi Weidong Department of Oncology, The Second People's Hospital of Nantong, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Chen Yuyan Class 5 Grade 13, Clinical Medicine, Medical College, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Xu Zhiwei Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Zhu Jia Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Zhang Tingting Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Huang Wei Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Ni Runzhou Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Lu Cuihua Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Zhang Xiubing Department of Oncology, The Second People's Hospital of Nantong, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 1-9 0300-8177 410:1-2<1 2015 410 11010 https://doi.org/10.1007/s11010-015-2533-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2533-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Shusen Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Shi Weidong Department of Oncology, The Second People's Hospital of Nantong, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yuyan Class 5 Grade 13, Clinical Medicine, Medical College, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Xu Zhiwei Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhu Jia Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Tingting Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Huang Wei Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Ni Runzhou Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Lu Cuihua Department of Digestion, Affiliated Hospital of Nantong University, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Xiubing Department of Oncology, The Second People's Hospital of Nantong, Nantong University, 226001, Nantong, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 410/1-2(2015-12-01), 1-9 0300-8177 410:1-2<1 2015 410 11010