caa a22 4500 606236074 CHVBK 20210128101246.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2426-y doi (NATIONALLICENCE)springer-10.1007/s11010-015-2426-y HDAC7 modulates TNF-α-mediated suppression of Leydig cell steroidogenesis [Elektronische Daten] [Mohanraj Sadasivam, Balamurugan Ramatchandirin, Sivasangari Balakrishnan, Chidambaram Prahalathan] The pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-α) has an inhibitory role in gonadal functions particularly in the steroidogenesis of Leydig cells. In the present study, we demonstrate that TNF-α activates histone deacetylases 7 (HDAC7), which regulates the expression of steroidogenic enzyme genes in Leydig cells. LC-540 Leydig cells were treated with TNF-α (10ng/ml) for different time intervals. TNF-α treatment significantly suppressed histone H3 acetylation and methylation and, concomitantly, increased the total histone deacetylases activity in LC-540 Leydig cells. RT-PCR and western blot analysis revealed that HDAC7 was up-regulated in TNF-α-treated cells. Our results also demonstrated that an siRNA-mediated knockdown of HDAC7 restores the expression of steroidogenic proteins in TNF-α-treated Leydig cells. These findings provide valuable information that TNF-α-mediated suppression of steroidogenesis involves HDAC7 in Leydig cells. Springer Science+Business Media New York, 2015 TNF-α nationallicence HDAC7 nationallicence Histone H3 modification nationallicence Steroidogenesis nationallicence Leydig cells nationallicence Sadasivam Mohanraj Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut Ramatchandirin Balamurugan Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut Balakrishnan Sivasangari Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut Prahalathan Chidambaram Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 83-90 0300-8177 406:1-2<83 2015 406 11010 https://doi.org/10.1007/s11010-015-2426-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2426-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sadasivam Mohanraj Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 700 1- Ramatchandirin Balamurugan Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 700 1- Balakrishnan Sivasangari Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 700 1- Prahalathan Chidambaram Department of Biochemistry, Bharathidasan University, 620 024, Tiruchirappalli, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 83-90 0300-8177 406:1-2<83 2015 406 11010