caa a22 4500 606236155 CHVBK 20210128101246.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2436-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2436-9 C-phycocyanin prevents cisplatin-induced mitochondrial dysfunction and oxidative stress [Elektronische Daten] [Berenice Fernández-Rojas, Daniela Rodríguez-Rangel, Luis Granados-Castro, Mario Negrette-Guzmán, Juan León-Contreras, Rogelio Hernández-Pando, Eduardo Molina-Jijón, José Reyes, Cecilia Zazueta, José Pedraza-Chaverri] The potential of C-phycocyanin (C-PC) to prevent cisplatin (CP)-induced kidney mitochondrial dysfunction was determined in CD-1 male mice. The CP-induced mitochondrial dysfunction was characterized by ultrastructural abnormalities and by decrease in the following parameters in isolated kidney mitochondria: adenosine diphosphate (ADP)-induced oxygen consumption (state 3), respiratory control ratio, ADP/oxygen (ADP/O) ratio, adenosine triphosphate synthesis, membrane potential, calcium retention, glutathione (GSH) content, and activity of respiratory complex I, aconitase, catalase, and GSH peroxidase. These mitochondria also showed increase in hydrogen peroxide production, malondialdehyde, and 3-nitrotyrosine protein adducts content. The above-described changes, as well as CP-induced nephrotoxicity, were attenuated in mice pretreated with a single injection of C-PC. Our data suggest that the attenuation of mitochondrial abnormalities is involved in the protective effect of C-PC against CP-induced nephrotoxicity. This is the first demonstration that C-PC pretreatment prevents CP-induced mitochondrial dysfunction in mice. Springer Science+Business Media New York, 2015 Functional food nationallicence C-phycocyanin nationallicence Mitochondrial oxygen consumption nationallicence Mitochondrial membrane potential nationallicence Nephrotoxicity nationallicence Cisplatin nationallicence Fernández-Rojas Berenice Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut Rodríguez-Rangel Daniela Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut Granados-Castro Luis Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut Negrette-Guzmán Mario Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut León-Contreras Juan Experimental Pathology Section, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán”, 14000, Mexico, D.F., Mexico aut Hernández-Pando Rogelio Experimental Pathology Section, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán”, 14000, Mexico, D.F., Mexico aut Molina-Jijón Eduardo Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), 07360, Mexico, Mexico aut Reyes José Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), 07360, Mexico, Mexico aut Zazueta Cecilia Department of Cardiovascular Biomedicine, Instituto Nacional de Cardiología "Ignacio Chávez”, 14080, Mexico, D.F., Mexico aut Pedraza-Chaverri José Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 183-197 0300-8177 406:1-2<183 2015 406 11010 https://doi.org/10.1007/s11010-015-2436-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2436-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fernández-Rojas Berenice Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Rodríguez-Rangel Daniela Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Granados-Castro Luis Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Negrette-Guzmán Mario Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- León-Contreras Juan Experimental Pathology Section, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán”, 14000, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Hernández-Pando Rogelio Experimental Pathology Section, Instituto Nacional de Ciencias Médicas y Nutrición "Salvador Zubirán”, 14000, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Molina-Jijón Eduardo Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), 07360, Mexico, Mexico aut NATIONALLICENCE 700 1- Reyes José Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute (Cinvestav-IPN), 07360, Mexico, Mexico aut NATIONALLICENCE 700 1- Zazueta Cecilia Department of Cardiovascular Biomedicine, Instituto Nacional de Cardiología "Ignacio Chávez”, 14080, Mexico, D.F., Mexico aut NATIONALLICENCE 700 1- Pedraza-Chaverri José Department of Biology, Facultad de Química, UNAM, Ciudad Universitaria, 04510, Mexico, D.F., Mexico aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 183-197 0300-8177 406:1-2<183 2015 406 11010