caa a22 4500 606236171 CHVBK 20210128101246.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2442-y doi (NATIONALLICENCE)springer-10.1007/s11010-015-2442-y Role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression, and induction of apoptosis in HeLa cells [Elektronische Daten] [Poorigali Sowmya, Bangalore Arathi, Kariyappa Vijay, Vallikannan Baskaran, Rangaswamy Lakshminarayana] The objective of the present study was to determine the role of different vehicles in carotenoids delivery and their influence on cell viability, cell cycle progression and induction of apoptosis in HeLa cells. Cells (5×103) were treated with different concentrations (25-100µM) of β-carotene (BC) or lutein (L) or astaxanthin (AST) dissolved in 0.5% of tetrahydrofuran (THF), dimethylsulfoxide (DMSO), and fetal bovine serum (FBS), respectively. The effect of delivery vehicle on carotenoids uptake, cytotoxicity, oxidative status, cell cycle distribution, and apoptosis was examined after 48h of incubation. The results shown that, cell viability reduced significantly in a dose- and time-dependent manner irrespective of carotenoid delivered in vehicles. Cellular uptake of BC delivered in THF was higher by 49.1, 29.7% and L delivered through THF was higher by 41.7 and 37.5% than DMSO and FBS, respectively. While, AST delivered through DMSO was higher by 36.1 and 43.7% than the THF and FBS, respectively. In case of cells treated either with BC or L delivered through THF and AST in DMSO decreased the glutathione and increased the malondialdehyde levels. The net increase in the G 2/M phase percentage of cell cycle progression was observed in carotenoid-treated cells. The % induction of apoptosis by BC or L delivered with THF and AST in DMSO was higher than other treated groups. In conclusion, choice of suitable vehicle for specific carotenoids delivery is essential that in turn may influence on cell proliferation and cell-based assays. Springer Science+Business Media New York, 2015 Carotenoids nationallicence Cytotoxicity nationallicence Cell cycle nationallicence Apoptosis nationallicence HeLa cells nationallicence AST : Astaxanthin nationallicence BC : β-Carotene nationallicence BHT : Butylated hydroxytoluene nationallicence DMSO : Dimethyl sulfoxide nationallicence DTNB : 5, 5-dithiobis (2-nitrobenzoic acid) nationallicence FBS : Fetal bovine serum nationallicence GR : Glutathione reductase nationallicence L : Lutein nationallicence MDA : Malondialdehyde nationallicence MEM : Minimal essential medium nationallicence MTT : 3-[4, 5-dimethylthiazol-2-yl]-2, 5- diphenyltetrazolium bromide nationallicence NADPH : Nicotinamide adenine dinucleotide phosphate oxidase nationallicence NEAA : Non-essential amino acid nationallicence THF : Tetrahydrofuran nationallicence TMP : Tetramethoxypropane nationallicence Sowmya Poorigali Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut Arathi Bangalore Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut Vijay Kariyappa Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut Baskaran Vallikannan Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, 570 020, Mysuru, India aut Lakshminarayana Rangaswamy Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 245-253 0300-8177 406:1-2<245 2015 406 11010 https://doi.org/10.1007/s11010-015-2442-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2442-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sowmya Poorigali Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut NATIONALLICENCE 700 1- Arathi Bangalore Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut NATIONALLICENCE 700 1- Vijay Kariyappa Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut NATIONALLICENCE 700 1- Baskaran Vallikannan Department of Biochemistry and Nutrition, CSIR-Central Food Technological Research Institute, 570 020, Mysuru, India aut NATIONALLICENCE 700 1- Lakshminarayana Rangaswamy Department of Biotechnology, Bangalore University, Jnana Bharathi Campus, 560 056, Bengaluru, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 245-253 0300-8177 406:1-2<245 2015 406 11010