caa a22 4500 606236201 CHVBK 20210128101246.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2420-4 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2420-4 MiR-34a promotes Fas-mediated cartilage endplate chondrocyte apoptosis by targeting Bcl-2 [Elektronische Daten] [Huajiang Chen, Jianxi Wang, Bo Hu, Xiaodong Wu, Yu Chen, Renhu Li, Wen Yuan] Apoptosis of cartilage endplate (CEP) chondrocytes is associated with the pathogenesis of intervertebral disk degeneration (IDD). Recent studies have shown that miR-34a is crucially involved in chondrocyte apoptosis during osteoarthritic cartilage. Here, we investigated the involvement of miR-34a in CEP chondrocyte apoptosis in IDD. In human degenerated CEP chondrocytes, miRNA (miR)-34a was markedly elevated in association with increased apoptosis. Bioinformatics target prediction identified Bcl-2 as a putative target of miR-34a. Furthermore, miR-34a inhibited Bcl-2 expression by directly targeting their 3′-untranslated regions, and this inhibition was abolished by mutation of the miR-34a binding sites. In vitro, knockdown of miR-34a in human endplate chondrocytes resulted in overexpression of Bcl-2, whereas upregulation of miR-34a led to repression of Bcl-2. Fas-mediated apoptosis was decreased when antagonizing miR-34a with locked nucleotide analog-miR-34a in human endplate chondrocytes. Taken together, our results demonstrate that upregulated miR-34a potentiates Fas-mediated endplate chondrocyte apoptosis, which is associated with IDD. Springer Science+Business Media New York, 2015 miR-34a nationallicence Cartilage endplate chondrocyte nationallicence Apoptosis nationallicence Bcl-2 nationallicence Fas nationallicence Chen Huajiang Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Wang Jianxi Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Hu Bo Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Wu Xiaodong Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Chen Yu Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Li Renhu Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Yuan Wen Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 21-30 0300-8177 406:1-2<21 2015 406 11010 https://doi.org/10.1007/s11010-015-2420-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2420-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Huajiang Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Jianxi Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Hu Bo Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xiaodong Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yu Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Li Renhu Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Yuan Wen Department of Spinal Surgery, Changzheng Hospital, Second Military Medical University, No. 415 Feng Yang Road, 200003, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 21-30 0300-8177 406:1-2<21 2015 406 11010