caa a22 4500 60623621X CHVBK 20210128101246.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2400-8 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2400-8 Hesperidin ameliorates trichloroethylene-induced nephrotoxicity by abrogation of oxidative stress and apoptosis in wistar rats [Elektronische Daten] [Aisha Siddiqi, Sana Nafees, Summya Rashid, Sarwat Sultana, Bano Saidullah] Trichloroethylene (TCE), a nephrotoxicant is known to cause severe damage to the kidney. In this study, the nephroprotective potential of hesperidin was evaluated against TCE-induced nephrotoxicity in wistar rats. Oral administration of TCE (1000mg/kg b.wt) for 15days enhanced renal lipid peroxidation and reduced antioxidant enzymes armoury viz., reduced renal glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, catalase and superoxide dismutase. It also enhanced the levels of blood urea nitrogen, creatinine and kidney injury molecule (KIM-1). Caspase-3 and bax expression were found to be elevated, while that of bcl-2 reduced suggesting that TCE induces apoptosis. However, pretreatment with hesperidin at a dose of 100 and 200mg/kg b.wt for 15days significantly decreased lipid peroxidation, increased the levels of antioxidant enzymes and reduced blood urea, creatinine and KIM-1 levels. Hesperidin also modulated the apoptotic pathways by altering the expressions of caspase-3, bax and bcl-2 to normal. Our results suggest that hesperidin can be used as a nephroprotective agent against TCE-induced nephrotoxicity. Graphical Abstract: Springer Science+Business Media New York, 2015 Trichloroethylene nationallicence Nephrotoxicity nationallicence Hesperidin nationallicence Oxidative stress nationallicence Apoptosis nationallicence TCE : 1,1,2-Trichloroethylene nationallicence DCVG : S-(1,2-dichlorovinyl)-l-glutathione nationallicence DCVC : S-(1,2-dichlorovinyl)-l-cysteine nationallicence GPx : Glutathione peroxidase nationallicence GSH : Glutathione nationallicence GR : Glutathione reductase nationallicence GST : Glutathione-S-transferase nationallicence LPO : Lipid peroxidation nationallicence MDA : Malondialdehyde nationallicence PMS : Post-mitochondrial supernatant nationallicence SOD : Superoxide dismutase nationallicence BUN : Blood urea nitrogen nationallicence KIM-1 : Kidney injury molecule nationallicence HS : Hesperidin nationallicence ROS : Reactive oxygen species nationallicence Siddiqi Aisha Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University, Maidan Garhi, 110068, New Delhi, India aut Nafees Sana Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, 110062, New Delhi, India aut Rashid Summya Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, 110062, New Delhi, India aut Sultana Sarwat Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, 110062, New Delhi, India aut Saidullah Bano Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University, Maidan Garhi, 110068, New Delhi, India aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 9-20 0300-8177 406:1-2<9 2015 406 11010 https://doi.org/10.1007/s11010-015-2400-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2400-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Siddiqi Aisha Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University, Maidan Garhi, 110068, New Delhi, India aut NATIONALLICENCE 700 1- Nafees Sana Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Rashid Summya Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Sultana Sarwat Section of Molecular Carcinogenesis and Chemoprevention, Department of Medical Elementology and Toxicology, Faculty of Science, Jamia Hamdard (Hamdard University), Hamdard Nagar, 110062, New Delhi, India aut NATIONALLICENCE 700 1- Saidullah Bano Discipline of Life Sciences, School of Sciences, Indira Gandhi National Open University, Maidan Garhi, 110068, New Delhi, India aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 9-20 0300-8177 406:1-2<9 2015 406 11010