caa a22 4500 606236260 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2444-9 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2444-9 Inhibitory effect of oblongifolin C on allergic inflammation through the suppression of mast cell activation [Elektronische Daten] [Yue Lu, Shuangfan Cai, Hongsheng Tan, Wenwei Fu, Hong Zhang, Hongxi Xu] Oblongifolin C (OC), a natural small molecule compound extracted from Garcinia yunnanensis Hu, has been previously shown to have anti-cancer effect, but the anti-allergic effect of OC has not yet been investigated. The aim of the present study is to determine the anti-allergic effect of OC on IgE/Ag-induced mouse bone marrow-derived mast cells (BMMCs) and on the passive systemic anaphylaxis (PSA) reaction in mice. OC clearly suppressed cyclooxygenase-2 (COX-2)-dependent prostaglandin D2 (PGD2) generation as well as leukotriene C4 (LTC4) generation and the degranulation reaction in IgE/Ag-stimulated BMMCs. Biochemical analyses of the IgE/Ag-mediated signaling pathways showed that OC suppressed the phosphorylation of phospholipase Cγ1 (PLCγ1)-mediated intracellular Ca2+ influx and the nuclear factor-κB (NF-κB) pathway, as well as the phosphorylation of mitogen-activated protein (MAP) kinases. Although OC did not inhibit the phosphorylation of Fyn, Lyn, and Syk, it directly inhibited the tyrosine kinase activity in vitro. Moreover, oral administration of OC inhibited the IgE-induced PSA reaction in a dose-dependent manner. Taken together, the present study provides new insights into the anti-allergic activity of OC, which could be a promising candidate for allergic therapy. Springer Science+Business Media New York, 2015 Oblongifolin C nationallicence Mouse bone marrow-derived mast cells nationallicence Nuclear factor- κ B nationallicence Tyrosine kinase activity nationallicence Passive systemic anaphylaxis nationallicence OC : Oblongifolin C nationallicence BMMCs : Bone marrow-derived mast cells nationallicence ERK1/2 : Extracellular signal regulated kinase1/2 nationallicence JNK : c-Jun N-terminal kinase nationallicence MAP Kinase : Mitogen-activated protein kinase nationallicence IKK : IκB kinase nationallicence Syk : Spleen tyrosine kinase nationallicence PLCγ1 : Phospholipase Cγ1 nationallicence HSA : Human serum albumin nationallicence LTC4 : Leukotriene C4 nationallicence PGD2 : Prostaglandin D2 nationallicence Lu Yue School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut Cai Shuangfan School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut Tan Hongsheng School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut Fu Wenwei School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut Zhang Hong School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut Xu Hongxi School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 263-271 0300-8177 406:1-2<263 2015 406 11010 https://doi.org/10.1007/s11010-015-2444-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2444-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lu Yue School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Cai Shuangfan School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Tan Hongsheng School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Fu Wenwei School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Hong School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Xu Hongxi School of Pharmacy, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 263-271 0300-8177 406:1-2<263 2015 406 11010