caa a22 4500 606236279 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2427-x doi (NATIONALLICENCE)springer-10.1007/s11010-015-2427-x Comparative analysis of sphingomyelin synthase 1 gene expression at the transcriptional and translational levels in human tissues [Elektronische Daten] [Olga Sudarkina, Ivan Filippenkov, Ilya Brodsky, Svetlana Limborska, Lyudmila Dergunova] Sphingomyelin synthase 1 (SMS1) catalyses the biosynthesis of sphingomyelin in eukaryotic cells. We have previously determined the structure of the SGMS1 gene encoding this enzyme and a number of its alternative transcripts. Here, we describe a study of the expression of the full-length SMS1 protein and the sum of the alternative transcripts encoding this protein in human tissues. The SMS1 protein and mRNA levels in tissues differed significantly and were not correlated, implying the active post-transcriptional regulation of SMS1 protein expression. The putative truncated isoforms of the SMS1 protein, which are encoded by a number of alternative transcripts, were not detected by immunoblotting and thus may be absent or present in only small amounts. Springer Science+Business Media New York, 2015 Human sphingomyelin synthase 1 nationallicence Gene expression nationallicence Transcript level nationallicence Immunoblotting nationallicence Protein detection nationallicence Real-time PCR nationallicence Sudarkina Olga Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut Filippenkov Ivan Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut Brodsky Ilya Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia aut Limborska Svetlana Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut Dergunova Lyudmila Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 91-99 0300-8177 406:1-2<91 2015 406 11010 https://doi.org/10.1007/s11010-015-2427-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 brief-communication jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2427-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sudarkina Olga Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut NATIONALLICENCE 700 1- Filippenkov Ivan Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut NATIONALLICENCE 700 1- Brodsky Ilya Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia aut NATIONALLICENCE 700 1- Limborska Svetlana Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut NATIONALLICENCE 700 1- Dergunova Lyudmila Human Molecular Genetics Department, Institute of Molecular Genetics, Russian Academy of Sciences, 123182, Moscow, Russia aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 91-99 0300-8177 406:1-2<91 2015 406 11010