caa a22 4500 606236287 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2438-7 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2438-7 Evidence for inhibition of nitric oxide and inducible nitric oxide synthase in Caco-2 and RAW 264.7 cells by a Maillard reaction product [5-(5,6-dihydro-4H-pyridin-3-ylidenemethyl)furan-2-yl]-methanol [Elektronische Daten] [Xiu-Min Chen, David Kitts] We have recently isolated and characterized the chemical structure of a bioactive Maillard reaction product, [5-(5,6-dihydro-4H-pyridin-3-ylidenemethyl)furan-2-yl]-methanol (F3-A), from an aqueous glucose (Glc) and lysine (Lys) Maillard reaction (MR) model system. Here, we investigate further the mechanisms for anti-inflammatory effects of this product in Caco-2 and RAW 264.7 cells. The anti-inflammatory capacity of F3-A recovered from Glc-Lys MR mixture and a synthesized product were compared with those of the specific inducible nitric oxide synthase (iNOS) inhibitor, aminoguanidine (AG), and the nuclear factor-kappa B (NF-κB) inhibitor, pyrrolidine dithiocarbamate(PDTC). F3-A produced a dose-dependent inhibition of extracellularnitric oxide (NO) production and iNOS translation in Caco-2 cells induced with interferon gamma(IFN-γ) and phorbol 12-myristate 13-acetate(PMA), and these effects were more potent than those obtained with AG. Moreover, a combination of F3-A and AG to attenuate intestinal inflammation was additive. However, F3-A inhibited only intracellular NO production in RAW 264.7 cells and did not inhibit COX-2 or NF-κB in either cell line. We conclude that the anti-inflammatory properties of F3-A are cell specific, working through different mechanism between macrophages and intestinal cells. Springer Science+Business Media New York, 2015 F3-A nationallicence Anti-inflammation nationallicence Intestinal diseases nationallicence Intestinal cell nationallicence Macrophage nationallicence AG : Aminoguanidine nationallicence COX-2 : Cyclooxygenase-2 nationallicence DAF-2 : 4,5-Diaminofluorescein nationallicence DAF-2DA : 4,5-Diaminofluorescein diacetate nationallicence DAF-2T : Triazolofluorescein nationallicence DCM : Dichloromethane nationallicence DMEM : Dulbecco's modified Eagle's medium nationallicence FBS : Fetal bovine serum nationallicence F3-A : [5-(5,6-Dihydro-4H-pyridin-3-ylidenemethyl)furan-2-yl]-methanol nationallicence F3-AC19 : Isolated F3-A purified by flash chromatography nationallicence F3-AS : Synthesized F3-A nationallicence Glc : Glucose nationallicence HMF : Hydroxymethylfurfural nationallicence HMFA : 5-Hydroxymethyl-2-furoic acid nationallicence IBD : Inflammatory bowel disease nationallicence IECs : Intestinal epithelial cells nationallicence IFN-γ : Interferon gamma nationallicence IL-6 : Interleukin-6 nationallicence IL-8 : Interleukin 8 nationallicence iNOS : Inducible nitric oxide synthase nationallicence LPS : Lipopolysaccharide nationallicence Lys : Lysine nationallicence 3-MCP : 3-Methyl-1,2-cyclopentanedione nationallicence MEM : Minimum essential medium nationallicence MR : Maillard reaction nationallicence MRPs : Maillard reaction products nationallicence MTT : 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide nationallicence NF-κB : Nuclear factor-kappa B nationallicence NMR : Nuclear magnetic resonance nationallicence NO : Nitric oxide nationallicence NO2 − : Nitrite nationallicence NO3 − : Nitrate nationallicence NOD : Nitric oxide dioxygenase nationallicence PDTC : Pyrrolidine dithiocarbamate nationallicence PMA : Phorbol 12-myristate 13-acetate nationallicence Rf : Retention factor nationallicence RT-PCR : Reverse transcription polymerase chain reaction nationallicence TLC : Thin layer chromatography nationallicence TBST : Tris-buffered saline-Tween nationallicence Chen Xiu-Min Food, Nutrition, and Health, Faculty of Land and Food Systems, University of British Columbia, 2205 East Mall, V6T 1Z4, Vancouver, BC, Canada aut Kitts David Food, Nutrition, and Health, Faculty of Land and Food Systems, University of British Columbia, 2205 East Mall, V6T 1Z4, Vancouver, BC, Canada aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 205-215 0300-8177 406:1-2<205 2015 406 11010 https://doi.org/10.1007/s11010-015-2438-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2438-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen Xiu-Min Food, Nutrition, and Health, Faculty of Land and Food Systems, University of British Columbia, 2205 East Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 700 1- Kitts David Food, Nutrition, and Health, Faculty of Land and Food Systems, University of British Columbia, 2205 East Mall, V6T 1Z4, Vancouver, BC, Canada aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 205-215 0300-8177 406:1-2<205 2015 406 11010