caa a22 4500 606236295 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2441-z doi (NATIONALLICENCE)springer-10.1007/s11010-015-2441-z ERK5 signalling pathway is essential for fluid shear stress-induced COX-2 gene expression in MC3T3-E1 osteoblast [Elektronische Daten] [Jin Jiang, Liang-gong Zhao, Yuan-jun Teng, Shao-long Chen, Li-ping An, Jing-ling Ma, Jing Wang, Ya-yi Xia] Bone cells respond to various mechanical stimuli including fluid shear stress (FSS) in vitro. Induction of cyclooxygenase-2 (COX-2) is thought to be important for the anabolic effects of mechanical loading. Recently, extracellular-signal-regulated kinase 5 (ERK5) has been found to be involved in multiple cellular processes. However, the relationship between ERK5 and the induction of COX-2 is still unknown. Here, we investigated the potential involvement of ERK5 in the response of pre-osteoblastic MC3T3-E1 cells upon FSS. MC3T3-E1 cells were subjected to 12 dyn/cm2 FSS. Then, we established a ERK5 small interfering RNA (siRNA) transfected cell line using the MC3T3-E1 cells. After the successful transfection confirmed by real-time reverse transcription-polymerase chain reaction and Western blotting, the expression of COX-2, cAMP response element-bindingprotein (CREB), and nuclear factor kappa B cells (NF-κB) were assayed for downstream effectors of activated ERK5 under FSS by Western blotting. Our results showed that FSS could stimulate COX-2 activity, and induce the phosphorylation of ERK5, CREB, and NF-κB. When the MC3T3-E1 cells were transfected using siRNA before exposure to FSS, COX-2 activity was suppressed, and the phosphorylation of CREB and NF-κB was significantly downregulated. In summary, we demonstrated that ERK5 pathway is essential in the induction of COX-2 gene. Springer Science+Business Media New York, 2015 Fluid shear stress nationallicence Osteoblast nationallicence Extracellular-signal-regulated kinase 5 (ERK5) nationallicence Cyclooxygenase-2 (COX-2) nationallicence Transcription factors nationallicence Jiang Jin Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut Zhao Liang-gong Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut Teng Yuan-jun Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut Chen Shao-long Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, Gansu, China aut An Li-ping Orthopaedics Key Laboratory of Gansu Province, Lanzhou, Gansu, China aut Ma Jing-ling Orthopaedics Key Laboratory of Gansu Province, Lanzhou, Gansu, China aut Wang Jing Orthopaedics Key Laboratory of Gansu Province, Lanzhou, Gansu, China aut Xia Ya-yi Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 237-243 0300-8177 406:1-2<237 2015 406 11010 https://doi.org/10.1007/s11010-015-2441-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2441-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jiang Jin Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- Zhao Liang-gong Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- Teng Yuan-jun Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- Chen Shao-long Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- An Li-ping Orthopaedics Key Laboratory of Gansu Province, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- Ma Jing-ling Orthopaedics Key Laboratory of Gansu Province, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- Wang Jing Orthopaedics Key Laboratory of Gansu Province, Lanzhou, Gansu, China aut NATIONALLICENCE 700 1- Xia Ya-yi Department of Orthopaedics, Second Hospital of Lanzhou University, No. 82 Cuiyingmen, Chengguan District, Lanzhou, Gansu, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 237-243 0300-8177 406:1-2<237 2015 406 11010