caa a22 4500 606236341 CHVBK 20210128101247.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11010-015-2421-3 doi (NATIONALLICENCE)springer-10.1007/s11010-015-2421-3 A FOXM1 related long non-coding RNA contributes to gastric cancer cell migration [Elektronische Daten] [Hui Cai, Jingde Chen, Bin He, Qiang Li, Yandong Li, Yong Gao] Long non-coding RNAs (LncRNAs) have been reported that play important roles in the progression and metastasis of some carcinomas. In the present study, we identified a new LncRNA, FRLnc1, from a microarray analysis in which those LncRNAs were regulated by FOXM1, an oncogene widely studied in most malignancies. Quantitative real-time PCR (qRT-PCR) results in gastric cancer cell lines indicated FRLnc1 expression is positively correlated with FOXM1 level, supporting the microarray data. Furthermore, the RNA level of FRLnc1 is upregulated in 49% (20/41) of cancer samples compared with neighboring non-cancerous stomach tissues. The in vitro functional analyses demonstrated that FRLnc1 knockdown by RNA interference suppressed cell migration in MGC803 and AGS cells, whereas FRLnc1 overexpression promoted cell migration in BGC823 and SGC7901 cells. Moreover, FRLnc1 could enhance the distant metastasis of SGC7901 cells by tail vein injection approach in mice. We also identified TGFβ1 and Twist as the downstream effectors of FRLnc1 in the regulation of cell migration by qRT-PCR analysis. Taken together, our findings suggest that FRLnc1 is involved in gastric cancer cell migration and for the first time set up the link between FOXM1 and LncRNA in cancer. Springer Science+Business Media New York, 2015 Gastric cancer nationallicence FOXM1 nationallicence LncRNA nationallicence Cell migration nationallicence Cai Hui Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut Chen Jingde Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut He Bin Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut Li Qiang Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut Li Yandong Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut Gao Yong Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 31-41 0300-8177 406:1-2<31 2015 406 11010 https://doi.org/10.1007/s11010-015-2421-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11010-015-2421-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai Hui Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut NATIONALLICENCE 700 1- Chen Jingde Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut NATIONALLICENCE 700 1- He Bin Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut NATIONALLICENCE 700 1- Li Qiang Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut NATIONALLICENCE 700 1- Li Yandong Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut NATIONALLICENCE 700 1- Gao Yong Department of Oncology, East Hospital, Tongji University School of Medicine, 150 Ji-Mo Road, 200120, Shanghai, China aut NATIONALLICENCE 773 0- Molecular and Cellular Biochemistry Springer US; http://www.springer-ny.com 406/1-2(2015-08-01), 31-41 0300-8177 406:1-2<31 2015 406 11010